Characterization of human immunodeficiency virus type 1 from a previously unexplored region of South Africa with a high HIV prevalence

被引:23
作者
Bessong, PO
Obi, CL
Cilliers, T
Choge, I
Phoswa, M
Pillay, C
Papathanasopoulos, M
Morris, L
机构
[1] Natl Inst Communicable Dis, AIDS Virus Res Unit, ZA-2131 Johannesburg, South Africa
[2] Univ Venda, Dept Microbiol, Thohoyandou, South Africa
关键词
D O I
10.1089/aid.2005.21.103
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
HIV prevalence in the Limpopo Province has increased rapidly within the past 10 years, as in other parts of South Africa. Little is known about the genetic and biological properties of HIV circulating in this region including the baseline drug resistance profiles. We therefore collected blood samples from 42 HIV- 1- infected patients residing in this region for analysis. All samples were shown to belong to HIV- 1 subtype C by env and gag heteroduplex mobility assay ( HMA). Viral isolates from 14 of these patients were shown to use the CCR5 coreceptor exclusively and had gp120 V3 loop sequences consistent with this phenotype. Sequence analysis of both protease and reverse transcriptase genes showed that none of 13 isolates harbored primary resistance mutations. These data suggest that HIV- 1 subtype C is the predominant subtype circulating in the Limpopo Province, and that viral strains from this region are indistinguishable from those found in other parts of South Africa.
引用
收藏
页码:103 / 109
页数:7
相关论文
共 22 条
[1]   ANALYSIS OF PARTIAL GAG AND ENV GENE-SEQUENCES OF HIV TYPE-1 STRAINS FROM SOUTHERN AFRICA [J].
BECKER, MLB ;
DEJAGER, G ;
BECKER, WB .
AIDS RESEARCH AND HUMAN RETROVIRUSES, 1995, 11 (10) :1265-1267
[2]   Genetic characterization of HIV type 1 from migrant workers in three South African gold mines [J].
Bredell, H ;
Williamson, C ;
Sonnenberg, P ;
Martin, DJ ;
Morris, L .
AIDS RESEARCH AND HUMAN RETROVIRUSES, 1998, 14 (08) :677-684
[3]   Identification of HIV type 1 intersubtype recombinants in South Africa using env and gag heteroduplex mobility assays [J].
Bredell, H ;
Hunt, G ;
Morgan, B ;
Tiemessen, CT ;
Martin, DJ ;
Morris, L .
AIDS RESEARCH AND HUMAN RETROVIRUSES, 2000, 16 (05) :493-497
[4]  
DELWART EL, 1995, PCR METH APPL, V4, pS202
[5]  
*DEP HLTH, 2001, NAT HIV SYPH SER SUR
[6]   HIV type 1 V3 domain serotyping and genotyping in Gauteng, Mpumalanga, KwaZulu-Natal, and Western Cape Provinces of South Africa [J].
Engelbrecht, S ;
Smith, TL ;
Kasper, P ;
Faatz, E ;
Zeier, M ;
Moodley, D ;
Clay, CG ;
Van Rensburg, EJ .
AIDS RESEARCH AND HUMAN RETROVIRUSES, 1999, 15 (04) :325-328
[7]   IDENTIFICATION OF ENV SUBTYPES IN 14 HIV TYPE-1 ISOLATES FROM SOUTH-AFRICA [J].
ENGELBRECHT, S ;
LATEN, JD ;
SMITH, TL ;
VANRENSBURG, EJ .
AIDS RESEARCH AND HUMAN RETROVIRUSES, 1995, 11 (10) :1269-1271
[8]   Molecular characteristics of human immunodeficiency virus type 1 subtype C viruses from KwaZulu-Natal, South Africa: Implications for vaccine and antiretroviral control strategies [J].
Gordon, M ;
De Oliveira, T ;
Bishop, K ;
Coovadia, HM ;
Madurai, L ;
Engelbrecht, S ;
van Rensburg, EJ ;
Mosam, A ;
Smith, A ;
Cassol, S .
JOURNAL OF VIROLOGY, 2003, 77 (04) :2587-2599
[9]   Simplified strategy for detection of recombinant human immunodeficiency virus type 1 group M isolates by gag/env heteroduplex mobility assay [J].
Heyndrickx, L ;
Janssens, W ;
Zekeng, L ;
Musonda, R ;
Anagonou, S ;
Van der Auwera, G ;
Coppens, S ;
Vereecken, K ;
De Witte, K ;
Van Rampelbergh, R ;
Kahindo, M ;
Morison, L ;
McCutchan, FE ;
Carr, JK ;
Albert, J ;
Essex, M ;
Goudsmit, J ;
Asjö, B ;
Salminen, M ;
Buvé, A ;
van der Groen, G .
JOURNAL OF VIROLOGY, 2000, 74 (01) :363-370
[10]  
KUSTNER HGV, 1994, S AFR MED J, V84, P195