Effect of liquid chromatography separation of complex matrices on liquid chromatography-tandem mass spectrometry signal suppression

被引：140

作者：

Choi, BK

Hercules, DM

Gusev, AI

机构：

[1] Rohm & Haas Co, Res Labs, Spring House, PA 19477 USA

[2] Vanderbilt Univ, Dept Chem, Nashville, TN 37235 USA

来源：

JOURNAL OF CHROMATOGRAPHY A | 2001年 / 907卷 / 1-2期

关键词：

signal suppression; liquid chromatography-mass spectrometry; hydroxyfenozide; methoxyfenozide; G-fenozide;

D O I：

10.1016/S0021-9673(00)01052-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The effect of liquid chromatography separation on liquid chromatogaphy-tandem mass spectrometry (LC-MS-MS) signal response for the characterization of low-molecular-mass compounds in a complex matrix was investigated. Matrix induced signal suppression appears throughout the entire LC-MS-MS analysis of wheat forage extract, with greatest suppression occurring at early retention times. Experimental results show that co-elution of matrix components and analytes from the LC column may be most strongly attributed to column overloading rather than similar analyte and matrix retention behavior. As a result, two-dimensional (LC-LC) separation can be a highly effective approach to address signal suppression effects for the quantitative LC-MS-MS analysis of complex matrix samples. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：337 / 342

页数：6

共 20 条

[1] ENHANCED SENSITIVITY FOR PEPTIDE-MAPPING WITH ELECTROSPRAY LIQUID-CHROMATOGRAPHY MASS-SPECTROMETRY IN THE PRESENCE OF SIGNAL SUPPRESSION DUE TO TRIFLUOROACETIC ACID-CONTAINING MOBILE PHASES [J].