Pseudomonas aeruginosa virulence genes identified in a Dictyostelium host model

被引：65

作者：

Alibaud, Laeticia ^{[1
]}

Koehler, Thilo ^{[2
,3
]}

Coudray, Alice ^{[1
]}

Prigent-Combaret, Claire ^{[1
]}

Bergeret, Evelyne ^{[4
,5
]}

Perrin, Jackie ^{[4
,5
]}

Benghezal, Mohammed ^{[1
]}

Reimmann, Cornelia ^{[6
]}

Gauthier, Yves ^{[7
]}

van Delden, Christian ^{[2
,3
]}

Attree, Ina ^{[8
]}

Fauvarque, Marie-Odile ^{[4
,5
]}

Cosson, Pierre ^{[1
]}

机构：

[1] Univ Geneva, Ctr Med Univ, Dept Physiol Cellulaire & Metab, CH-1211 Geneva 4, Switzerland

[2] Univ Geneva, Ctr Med Univ, Dept Microbiol & Med Mol, CH-1211 Geneva, Switzerland

[3] Univ Hosp Geneva, Serv Malad Infect, CH-1211 Geneva, Switzerland

[4] iRTSV LTS, DSV, CEA, F-38054 Grenoble, France

[5] Univ Grenoble 1, F-38041 Grenoble, France

[6] Univ Lausanne, Dept Microbiol Fondamentale, CH-1015 Lausanne, Switzerland

[7] Ctr Rech Serv Sante Armees, Dept Biol Agents Transmissibles, F-38702 La Tronche, France

[8] UJF, iRTSV LBBSI, CEA, CNRS, F-75700 Paris, France

来源：

CELLULAR MICROBIOLOGY | 2008年 / 10卷 / 03期

关键词：

D O I：

10.1111/j.1462-5822.2007.01080.x

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The human pathogen Pseudomonas aeruginosa has been shown previously to use similar virulence factors when infecting mammalian hosts or Dictyostelium amoebae. Here we randomly mutagenized a clinical isolate of P. aeruginosa, and identified mutants with attenuated virulence towards Dictyostelium. These mutant strains also exhibited a strong decrease in virulence when infecting Drosophila and mice, confirming that P. aeruginosa makes use of similar virulence traits to confront these very different hosts. Further characterization of these bacterial mutants showed that TrpD is important for the induction of the quorum-sensing circuit, while PchH and PchI are involved in the induction of the type III secretion system. These results demonstrate the usefulness and the relevance of the Dictyostelium host model to identify and analyse new virulence genes in P. aeruginosa.

引用

页码：729 / 740

页数：12

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