Postexercise muscle glycogen resynthesis in obese insulin-resistant Zucker rats

被引:15
作者
Bruce, CR [1 ]
Lee, JS [1 ]
Hawley, JA [1 ]
机构
[1] RMIT Univ, Sch Med Sci, Fac Life Sci, Exercise Metab Grp, Bundoora, Vic 3083, Australia
关键词
glycogen synthase; GLUT-4; hexokinase; insulin resistance;
D O I
10.1152/jappl.2001.91.4.1512
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
We determined the effect of an acute bout of swimming (8 x 30 min) followed by either carbohydrate administration (0.5 mg/g glucose ip and ad libitum access to chow; CHO) or fasting (Fast) on postexercise glycogen resynthesis in soleus muscle and liver from female lean (ZL) and obese insulin-resistant (ZO) Zucker rats. Resting soleus muscle glycogen concentration ([glycogen]) was similar between genotypes and was reduced by 73 (ZL) and 63% (ZO) after exercise (P < 0.05). Liver [glycogen) at rest was greater in ZO than ZL (334 +/- 31 vs. 247 +/- 16 <mu>mol/g wet wt; P < 0.01) and fell by 44 and 94% after exercise (P < 0.05). The fractional activity of glycogen synthase (active/total) increased immediately after exercise (from 0.22 +/- 0.05 and 0.32 +/- 0.04 to 0.63 +/- 0.08 vs. 0.57 +/- 0.05; P < 0.01 for ZL and ZO rats, respectively) and remained elevated above resting values after 30 min of recovery. During this time, muscle [glycogen] in ZO increased 68% with CHO (P < 0.05) but did not change in Fast. Muscle [glycogen] was unchanged in ZL from postexercise values after both treatments. After 6 h recovery, GLUT-4 protein concentration was increased above resting levels by a similar extent for both genotypes in both fasted (similar to 45%) and CHO-supplemented (similar to 115%) rats. Accordingly, during this time CHO refeeding resulted in supercompensation in both genotypes (68% vs. 44% for ZL and ZO). With CHO, liver [glycogen] was restored to resting levels in ZL but remained at postexercise values for ZO after both treatments. We conclude that the increased glucose availability with carbohydrate refeeding after glycogen-depleting exercise resulted in glycogen supercompensation, even in the face of muscle insulin-resistance.
引用
收藏
页码:1512 / 1519
页数:8
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