Minocycline up-regulates BCL-2 levels in mitochondria and attenuates male germ cell apoptosis

被引：33

作者：

Castanares, M

Vera, Y

Erkkilä, K

Kyttänen, S

Lue, YH

Dunkel, L

Wang, C

Swerdloff, RS

Hikim, APS ^{[1
]}

机构：

[1] Harbor UCLA Med Ctr, Dept Med, Div Endocrinol, Torrance, CA 90509 USA

[2] Univ Calif Los Angeles, Los Angeles Biomed Res Inst, David Geffen Sch Med, Torrance, CA 90509 USA

[3] Biomedicum, Program Dev & Reprod Biol, FIN-00029 Helsinki, Finland

[4] Univ Helsinki, Hosp Children & Adolescents, FIN-00029 Helsinki, Finland

[5] Kuopio Univ Hosp, Dept Pediat, FIN-70211 Kuopio, Finland

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2005年 / 337卷 / 02期

关键词：

apoptosis; hormone withdrawal; minocycline; BCL-2; cytochrome c; DIABLO; germ cells; testis;

D O I：

10.1016/j.bbrc.2005.09.101

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study, we determined the efficacy of minocycline, a second generation tetracycline, in preventing male germ cell apoptosis after withdrawal of gonadotropins and intratesticular testosterone (T). Groups of 5 male rats received one of the following treatments daily for 5 days: (i) daily sc injection of GnRH-A (1.6 mg/kg BW), (ii) oral administration of 30% gum acacia as a vehicle control, and (iii) GnRH-A + oral administration of 50 or 100 mg/kg BW of minocycline. Minocycline at both 50 and 100 mg dose levels significantly (P < 0.05) prevented GnRH-A-induced germ cell apoptosis by 59.4% and 62.2%, respectively, and fully prevented PARP cleavage. Minocycline-mediated protection occurred at the mitochondria, involving the restoration of the BCL-2 levels and, in turn, suppression of cytochrome c and DIABLO release. Minocycline was also effective in preventing human male germ cell apoptosis induced by hormone free culture condition. (c) 2005 Elsevier Inc. All rights reserved.

引用

页码：663 / 669

页数：7

共 34 条

[1] Minocycline markedly protects the neonatal brain against hypoxic-ischemic injury [J].