Sequencing our way towards understanding global eukaryotic biodiversity

被引:317
作者
Bik, Holly M. [1 ]
Porazinska, Dorota L. [2 ]
Creer, Simon [3 ]
Caporaso, J. Gregory [4 ]
Knight, Rob [5 ,6 ]
Thomas, W. Kelley [1 ]
机构
[1] Univ New Hampshire, Hubbard Ctr Genome Studies, Durham, NH 03824 USA
[2] Univ Florida, Ft Lauderdale Res & Educ Ctr, IFAS, Ft Lauderdale, FL 33314 USA
[3] Bangor Univ, Sch Biol Sci, Environm Ctr Wales, Coll Nat Sci, Bangor LL57 2UW, Gwynedd, Wales
[4] No Arizona Univ, Dept Comp Sci, Flagstaff, AZ 86011 USA
[5] Univ Colorado, Dept Chem & Biochem, Boulder, CO 80309 USA
[6] Univ Colorado, Howard Hughes Med Inst, Boulder, CO 80309 USA
基金
美国国家科学基金会; 英国自然环境研究理事会; 美国国家卫生研究院;
关键词
DEEP-SEA; MICROBIAL DIVERSITY; SPECIES RICHNESS; AMPLICON READS; RARE BIOSPHERE; DNA; COMMUNITY; PCR; RESOURCE; REVEALS;
D O I
10.1016/j.tree.2011.11.010
中图分类号
Q14 [生态学(生物生态学)];
学科分类号
071012 ; 0713 ;
摘要
Microscopic eukaryotes are abundant, diverse and fill critical ecological roles across every ecosystem on Earth, yet there is a well-recognized gap in understanding of their global biodiversity. Fundamental advances in DNA sequencing and bioinformatics now allow accurate en masse biodiversity assessments of microscopic eukaryotes from environmental samples. Despite a promising outlook, the field of eukaryotic marker gene surveys faces significant challenges: how to generate data that are most useful to the community, especially in the face of evolving sequencing technologies and bioinformatics pipelines, and how to incorporate an expanding number of target genes.
引用
收藏
页码:233 / 243
页数:11
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