Administration of poly[di(sodium carboxylatoethylphenoxy)]phosphazene (PCEP) as adjuvant activated mixed Th1/Th2 immune responses in pigs

被引：23

作者：

Dar, Arshud ^{[1
]}

Lai, Ken ^{[1
]}

Dent, Donna ^{[1
]}

Potter, Andrew ^{[1
]}

Gerdts, Volker ^{[1
]}

Babiuk, Lorne A. ^{[2
]}

Mutwiri, George K. ^{[1
,3
]}

机构：

[1] Univ Saskatchewan, Dept Vet Microbiol, Vaccine & Infect Dis Org, Saskatoon, SK S7N 5E3, Canada

[2] Univ Alberta, Edmonton, AB T6G 2J9, Canada

[3] Univ Saskatchewan, Sch Publ Hlth, Saskatoon, SK S7N 5E3, Canada

来源：

VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY | 2012年 / 146卷 / 3-4期

关键词：

Adjuvants; A.pleuropneumoniae; Humoral immune responses; Cellular immune responses; OUTER-MEMBRANE LIPOPROTEIN; INJECTION SITE LESIONS; VIRAL-DIARRHEA-VIRUS; ACTINOBACILLUS-PLEUROPNEUMONIAE; CPG OLIGODEOXYNUCLEOTIDES; PROTECTIVE IMMUNITY; ALUMINUM ADJUVANTS; VACCINE ADJUVANTS; MICE; POLYPHOSPHAZENES;

D O I：

10.1016/j.vetimm.2012.01.021

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The selection of an optimal adjuvant to enhance the potency and longevity of antigen specific immune responses has always been imperative for the development of more effective and safer vaccines. A balanced type of immune enhancing ability of a new adjuvant known as polyphosphazene (PCEP) has been demonstrated in mice. In the present study we have compared the humoral and cellular immune responses to vaccine formulations containing Actinobacillus pleuropneumoniae outer membrane antigen (OmlA) with PCEP or Emulsigen (R) as adjuvants. Our data showed a significant improvement and a shift toward more balanced immune responses when OmlA antigen was formulated with PCEP and CpG ODN. Moreover, in contrast to Emulsigen (R), immunization with PCEP did not result in local injection site reactions highlighting its potential as a safe and effective adjuvant for pigs. Further, the ease of formulation, administration and relatively low per dose cost of PCEP make it a promising adjuvant for pigs. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：289 / 295

页数：7

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