Intracellular ionic strength regulates the volume sensitivity of a swelling-activated anion channel

Cell swelling activates an outwardly rectifying anion channel termed VSOAC (volume-sensitive organic osmolyte/anion channel). Regulation of VSOAC by intracellular electrolytes was characterized in Chinese hamster ovary cells by whole cell patch clamp. Elevation of intracellular CsCl concentration from 40 to 180 mM resulted in a concentration-dependent decrease in channel activation. Activation of VSOAC was insensitive to the salt gradient across the plasma membrane, the intracellular concentration of specific anions or cations, and the total intracellular concentration of cations, anions, or electrolytes. Comparison of cells dialyzed with either CsCl or Na2SO4 solutions demonstrated directly that VSOAC activation is modulated by intracellular ionic strength (mu(i)) The relative cell volume at which VSOAC current activation was triggered, termed the channel volume set point, decreased with decreasing ionic strength. At mu(i) = 0.04, VSOAC activation occurred spontaneously in shrunken cells. The rate of VSOAC activation mras nearly 50-fold higher in cells with mu(i) = 0.04 vs. those with mu(i) = 0.18. We propose that mu(i) modulates the volume sensor responsible for channel activation.