Recombinational DNA double-strand breaks in mice precede synapsis

被引：713

作者：

Mahadevaiah, SK

Turner, JMA

Baudat, F

Rogakou, EP

de Boer, P

Blanco-Rodríguez, J

Jasin, M

Keeney, S

Bonner, WM

Burgoyne, PS

机构：

[1] Natl Inst Med Res, Div Dev Genet, London NW7 1AA, England

[2] Mem Sloan Kettering Canc Ctr, New York, NY 10021 USA

[3] NCI, Mol Pharmacol Lab, NIH, Bethesda, MD 20892 USA

[4] Wageningen Inst Anim Sci, Genet Lab, Wageningen, Netherlands

[5] Univ Valladolid, Sch Med, Dept Cell Biol, Valladolid, Spain

来源：

NATURE GENETICS | 2001年 / 27卷 / 03期

关键词：

D O I：

10.1038/85830

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

In Saccharomyces cerevisiae, meiotic recombination is initiated by Spo11-dependent double-strand breaks (DSBs), a process that precedes homologous synapsis. Here we use an antibody specific for a phosphorylated histone (gamma -H2AX, which marks the sites of DSBs) to investigate the timing, distribution and Spo11-dependence of meiotic DSBs in the mouse. We show that, as in yeast, recombination in the mouse is initiated by Spo11-dependent DSBs that form during leptotene. Loss of gamma -H2AX staining (which in irradiated somatic cells is temporally linked with DSB repair) is temporally and spatially correlated with synapsis, even when this synapsis is 'non-homologous'.

引用

页码：271 / 276

页数：6

共 36 条

[1] Change of karyoskeleton during mammalian spermatogenesis: Expression pattern of nuclear lamin C2 and its regulation [J].