Expanding autologous multipotent mesenchymal bone marrow stromal cells

被引：23

作者：

Brinchmann, Jan E. ^{[1
]}

机构：

[1] Univ Oslo, Rikshosp, Med Ctr, Inst Immunol, N-0027 Oslo, Norway

来源：

JOURNAL OF THE NEUROLOGICAL SCIENCES | 2008年 / 265卷 / 1-2期

关键词：

mesenchymal stem cells; multipotent mesenchymal stromal cells; immunosuppressive cells; immunoprotected cells; in vitro cell culture; PROLIFERATION IN-VITRO; VERSUS-HOST-DISEASE; STEM-CELLS; INTERNATIONAL-SOCIETY; CULTURE-CONDITIONS; EXPANSION; THERAPY; TISSUE; OSTEOGENESIS; INHIBIT;

D O I：

10.1016/j.jns.2007.05.006

中图分类号：

R74 [神经病学与精神病学];

学科分类号：

摘要：

in this brief review, I will start by redefining the cells: mesenchymal stem cells should now be called multipotent mesenchymal stromal cells, with the same acronym (MSC). I will review the role of MSC as immunosuppressive and immunoprotected cells, and then go on to describe how cell culture strategies may depend on the desired functionality of MSC. In order to expand MSC in vitro, the cells have to be cultured as adherent cells on plastic surfaces. To obtain the best cell culture conditions, a number of methodological decisions have to be made. Special considerations are necessary if the cells are to be used for the treatment of patients. (C) 2007 Elsevier B.V. All rights reserved.

引用

页码：127 / 130

页数：4

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