Differences in the composition of hip and knee synovial fluid in osteoarthritis: a nuclear magnetic resonance (NMR) spectroscopy study of metabolic profiles

被引:11
作者
Akhbari, P. [1 ]
Jaggard, M. K. [1 ]
Boulange, C. L. [2 ]
Vaghela, U. [3 ]
Graca, G. [2 ]
Bhattacharya, R. [1 ]
Lindon, J. C. [2 ]
Williams, H. R. T. [4 ]
Gupte, C. M. [1 ]
机构
[1] Imperial Coll Healthcare NHS Trust, Dept Orthopaed & Trauma, London, England
[2] Imperial Coll Healthcare NHS Trust, Dept Digest Dis, London, England
[3] Imperial Coll London, Dept Metab Digest & Reprod, London, England
[4] Imperial Coll London, Sch Med, London, England
关键词
Metabolic profiling; Metabonomics; NMR; Osteoarthritis; Synovial fluid; EXTRACELLULAR-MATRIX; SERUM; REVEALS; IDENTIFICATION; PROTEOGLYCANS; ASSOCIATION; INFECTION; CARTILAGE; PLASMA; ACID;
D O I
10.1016/j.joca.2019.07.017
中图分类号
R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学(修复外科学)];
学科分类号
100224 [整形外科学];
摘要
Objective: The hip and knee joints differ biomechanically in terms of contact stresses, fluid lubrication and wear patterns. These differences may be reflected in the synovial fluid (SF) composition of the two joints, but the nature of these differences remains unknown. The objective was to identify differences in osteoarthritic hip and knee SF metabolites using metabolic profiling with Nuclear Magnetic Resonance (NMR) spectroscopy. Design: Twenty-four SF samples (12 hip, 12 knee) were collected from patients with end-stage osteoarthritis (ESOA) undergoing hip/knee arthroplasty. Samples were matched for age, gender, ethnicity and had similar medical comorbidities. NMR spectroscopy was used to analyse the metabolites present in each sample. Principal Component Analysis and Orthogonal Partial Least Squares Discriminant Analysis were undertaken to investigate metabolic differences between the groups. Metabolites were identified using 2D NMR spectra, statistical spectroscopy and by comparison to entries in published databases. Results: There were significant differences in the metabolic profile between the groups. Four metabolites were found in significantly greater quantities in the knee group compared to the hip group (N-acetylated molecules, glycosaminoglycans, citrate and glutamine). Conclusions: This is the first study to indicate differences in the metabolic profile of hip and knee SF in ESOA. The identified metabolites can broadly be grouped into those involved in collagen degradation, the tricarboxylic acid cycle and oxidative metabolism in diseased joints. These findings may represent a combination of intra and extra-articular factors. (C) 2019 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码:1768 / 1777
页数:10
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共 50 条
[1]
The Role of Metabolomics in Osteoarthritis Research [J].
Adams, Samuel B., Jr. ;
Setton, Lori A. ;
Nettles, Dana L. .
JOURNAL OF THE AMERICAN ACADEMY OF ORTHOPAEDIC SURGEONS, 2013, 21 (01) :63-64
[2]
Gait Mechanics Influence Healthy Cartilage Morphology and Osteoarthritis of the Knee [J].
Andriacchi, Thomas P. ;
Koo, Seungbum ;
Scanlan, Sean F. .
JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME, 2009, 91A :95-101
[3]
[Anonymous], 2015, Oxidative Phosphorylation
[4]
Statistical methods for the analysis of high-throughput metabolomics data [J].
Bartel, Joerg ;
Krumsiek, Jan ;
Theis, Fabian J. .
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL, 2013, 4 (05)
[5]
CONTROLLING THE FALSE DISCOVERY RATE - A PRACTICAL AND POWERFUL APPROACH TO MULTIPLE TESTING [J].
BENJAMINI, Y ;
HOCHBERG, Y .
JOURNAL OF THE ROYAL STATISTICAL SOCIETY SERIES B-STATISTICAL METHODOLOGY, 1995, 57 (01) :289-300
[6]
Mitochondrial dysfunction in osteoarthritis [J].
Blanco, FJ ;
López-Armada, MJ ;
Maneiro, E .
MITOCHONDRION, 2004, 4 (5-6) :715-728
[7]
Aspartic acid racemization reveals a high turnover state in knee compared with hip osteoarthritic cartilage [J].
Catterall, J. B. ;
Zura, R. D. ;
Bolognesi, M. P. ;
Kraus, V. B. .
OSTEOARTHRITIS AND CARTILAGE, 2016, 24 (02) :374-381
[8]
The Global Economic Cost of Osteoarthritis: How the UK Compares [J].
Chen, A. ;
Gupte, C. ;
Akhtar, K. ;
Smith, P. ;
Cobb, J. .
ARTHRITIS, 2012,
[9]
Statistical total correlation spectroscopy:: An exploratory approach for latent biomarker identification from metabolic 1H NMR data sets [J].
Cloarec, O ;
Dumas, ME ;
Craig, A ;
Barton, RH ;
Trygg, J ;
Hudson, J ;
Blancher, C ;
Gauguier, D ;
Lindon, JC ;
Holmes, E ;
Nicholson, J .
ANALYTICAL CHEMISTRY, 2005, 77 (05) :1282-1289
[10]
Comparative study of normal and osteoarthritic canine synovial fluid using 500 MHz 1H magnetic resonance spectroscopy [J].
Damyanovich, AZ ;
Staples, JR ;
Chan, ADM ;
Marshall, KW .
JOURNAL OF ORTHOPAEDIC RESEARCH, 1999, 17 (02) :223-231