Development of colloidal gold-based flow-through and lateral-flow immunoassays for the rapid detection of the insecticide carbaryl

被引：76

作者：

Wang, S ^{[1
]}

Zhang, C ^{[1
]}

Wang, JP ^{[1
]}

Zhang, Y ^{[1
]}

机构：

[1] Tianjin Univ, Fac Food Engn & Biotechnol, Tianjin Key Lab Food Nutr & Safety, Tianjin 300222, Peoples R China

来源：

ANALYTICA CHIMICA ACTA | 2005年 / 546卷 / 02期

关键词：

carbaryl; flow-through; lateral-flow; colloidal gold-based immunoassay;

D O I：

10.1016/j.aca.2005.04.088

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

One-step membrane-based competitive colloidal gold-based immunoassays in flow-through and lateral-flow formats for the rapid detection of carbaryl were developed. Nitro-cellulose membrane strip was separately coated with goat anti-rabbit IgG (control line) and carbaryl hapten-OVA conjugate (test line). Anti-carbaryl antibody labeled with colloidal gold particles was firstly incubated with carbaryl. A positive reaction as a result of the remaining antibody-gold conjugate combining with antigen coated on the membrane was obvious by visual detection, with detection limits for flow-through and lateral flow of 50 and 100 mu g/L, respectively. The assay time for both tests was less than 5 min, suitable for rapid testing on-site. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：161 / 166

页数：6

共 20 条

[1] APPLICATION OF A MONOCLONAL ANTIBODY-BASED ELISA TO THE DETERMINATION OF CARBARYL IN APPLE AND GRAPE JUICES
ABAD, A
MONTOYA, A
[J]. ANALYTICA CHIMICA ACTA, 1995, 311 (03) : 365 - 370
[2] Monoclonal enzyme immunoassay for the analysis of carbaryl in fruits and vegetables without sample cleanup
Abad, A
Moreno, MJ
Pelegrí, R
Martínez, MI
Sáez, A
Gamón, M
Montoya, A
[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2001, 49 (04) : 1707 - 1712
[3] Aga DS, 1997, ACS SYM SER, V657, P1
[4] *BANGS LAB INC, 1997, SYLL LAT COURS LOND
[5] BRIAN D, 1993, J CHROMATOGR, V642, P89
[6] An electrochemical metalloimmunoassay based on a colloidal gold label
Dequaire, M
Degrand, C
Limoges, B
[J]. ANALYTICAL CHEMISTRY, 2000, 72 (22) : 5521 - 5528
[7] Dykman LA, 1997, BIOCHEMISTRY-MOSCOW+, V62, P350
[8] CONTROLLED NUCLEATION FOR REGULATION OF PARTICLE-SIZE IN MONODISPERSE GOLD SUSPENSIONS
FRENS, G
[J]. NATURE-PHYSICAL SCIENCE, 1973, 241 (105): : 20 - 22
[9] HAYAT MA, 1989, COLLOIDAL GOLD PRINC, P1519
[10] HERMANSON GT, 1996, BIOCONJUGATE TECHNIQ

← 1 2 →