Production of hyaluronan and chondroitin sulphate proteoglycans from human arterial smooth muscle - the effect of glucose, insulin, IGF-1 or growth hormone

被引:38
作者
Erikstrup, C
Pedersen, LM
Heickendorff, L
Ledet, T
Rasmussen, LM
机构
[1] Univ Aarhus, Res Lab Biochem Pathol, Aarhus Kommune Hosp, DK-8000 Aarhus C, Denmark
[2] Univ Aarhus, Inst Expt Clin Res, Aarhus Kommune Hosp, Mol Biol Lab, DK-8000 Aarhus, Denmark
[3] Aarhus Kommune Hosp, Dept Clin Biochem, Aarhus, Denmark
关键词
D O I
10.1530/eje.0.1450193
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Background: Although it is recognized that the extracellular matrix is important for cell proliferation, migration and metabolism of growth factors, the regulation of the synthesis of hyaluronan and chondroitin sulphate proteoglycan (CSPG) in the vessel wall is poorly understood. Objective: To examine the role of glucose, insulin, IGF-I and human growth hormone (hGH) on the accumulation of hyaluronan and CSPG using cultures of human aortic smooth muscle cells. Methods: The cultures were exposed for 36 h. The CSPG content in the incubation medium was measured by a combination of digestion with testicular hyaluronidase and precipitation of [(SO42-)-S-35]-labelled material with ethanol and trichloroacetic acid. Hyaluronan was estimated using a radiometric assay. Results: Glucose and insulin reduced the amount of synthesized hyaluronan (2P < 0.01). Stimulation of synthesis was seen with hGH (2P < 0.01), whereas no effect was observed with IGF-I. The production of CSPG was increased with glucose and hGH (2P < 0.01), but showed no change with insulin. Conclusions: The present data obtained with human arterial smooth muscle cells in vitro showed that glucose, insulin and hGH can influence the accumulation of hyaluronan and CSPG. These observations may be relevant for an understanding of diabetic macroangiopathy.
引用
收藏
页码:193 / 198
页数:6
相关论文
共 35 条
[1]   Insulin-like growth factor binding proteins-2 to-6 are expressed by human vascular smooth muscle cells [J].
Andersson, P ;
Gustafsson, T ;
Arnqvist, HJ .
JOURNAL OF ENDOCRINOLOGY, 1999, 163 (02) :281-288
[2]  
BASSOLS A, 1988, J BIOL CHEM, V263, P3039
[3]   EFFECT OF INSULIN ON SULFATED PROTEOGLYCAN SYNTHESIS IN CULTURED SMOOTH-MUSCLE CELLS FROM PIG AORTA [J].
BRETON, M ;
BERROU, E ;
DEUDON, E ;
BRAHIMIHORN, MC ;
PICARD, J .
EXPERIMENTAL CELL RESEARCH, 1988, 177 (01) :212-220
[4]   Effects of diabetes and insulin treatment of diabetic rats on hyaluronan and hyaluronectin production in injured aorta [J].
Chajara, A ;
Raoudi, M ;
Delpech, B ;
Courel, MN ;
Leroy, M ;
Basuyau, JP ;
Levesque, H .
JOURNAL OF VASCULAR RESEARCH, 1999, 36 (03) :209-221
[5]   Perlecan and basement membrane chondroitin sulfate proteoglycan (Bamacan) are two basement membrane chondroitin dermatan sulfate proteoglycans in the Engelbreth-Holm-Swarm tumor matrix [J].
Couchman, JR ;
Kapoor, R ;
Sthanam, M ;
Wu, RR .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (16) :9595-9602
[6]  
DYBDAHL H, 1987, DIABETOLOGIA, V30, P882
[7]   Regulation of IGF binding proteins in human aorta vascular smooth muscle cells by cAMP, dexamethasone and IGF-I [J].
Hayford, K ;
Boes, M ;
Dake, BL ;
Bar, RS .
GROWTH HORMONE & IGF RESEARCH, 1998, 8 (05) :369-375
[8]   GLYCOSAMINOGLYCANS OF ARTERIAL BASEMENT MEMBRANE-LIKE MATERIAL FROM CULTURED RABBIT AORTIC MYOMEDIAL CELLS [J].
HEICKENDORFF, L ;
LEDET, T .
BIOCHIMICA ET BIOPHYSICA ACTA, 1984, 798 (02) :276-282
[9]   GLYCOSAMINOGLYCANS IN THE HUMAN AORTA IN DIABETES-MELLITUS - A STUDY OF TUNICA MEDIA FROM AREAS WITH AND WITHOUT ATHEROSCLEROTIC PLAQUE [J].
HEICKENDORFF, L ;
LEDET, T ;
RASMUSSEN, LM .
DIABETOLOGIA, 1994, 37 (03) :286-292
[10]  
HEINEGARD D, 1987, STRUCTURAL CONTRACTI, P319