Selection and analytical applications of aptamers binding microbial pathogens

被引：107

作者：

Hamula, Camille L. A. ^{[1
]}

Zhang, Hongquan ^{[1
]}

Li, Feng ^{[1
]}

Wang, Zhixin ^{[1
]}

Le, X. Chris ^{[1
]}

Li, Xing-Fang ^{[1
]}

机构：

[1] Univ Alberta, Div Analyt & Environm Toxicol, Dept Lab Med & Pathol, Fac Med & Dent, Edmonton, AB T6G 2G3, Canada

来源：

TRAC-TRENDS IN ANALYTICAL CHEMISTRY | 2011年 / 30卷 / 10期

基金：

加拿大自然科学与工程研究理事会; 加拿大健康研究院;

关键词：

Aptamer; Bacteria; Bioanalytical assay; Biosensor; DNA; In-vitro selection; Pathogen; Protein; SELEX; SOMAmer; HEPATITIS-C VIRUS; IN-VITRO SELECTION; RNA APTAMERS; DNA APTAMERS; SSDNA APTAMERS; PRION-PROTEIN; COMBINATORIAL SELECTION; SURFACE; ASSAY; POLYMERASE;

D O I：

10.1016/j.trac.2011.08.006

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

DNA aptamers specifically recognizing microbial cells and viruses have a range of analytical and therapeutic applications. This article describes recent advances in the development of aptamers targeting specific pathogens (e.g., live bacteria, whole viral particles, and virally-infected mammalian cells). Specific aptamers against pathogens have been used as affinity reagents to develop sandwich assays, to label and to image cells, to bind with cells for flow-cytometry analysis, and to act as probes for development of whole-cell biosensors. Future applications of aptamers to pathogens will benefit from recent advances in improved selection and new aptamers containing modified nucleotides, particularly slow off-rate modified aptamers (SOMAmers). (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：1587 / 1597

页数：11

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