Investigation of the electrospray plume by laser-induced fluorescence spectroscopy

被引：64

作者：

Zhou, SL

Edwards, AG

Cook, KD ^{[1
]}

Van Berkel, GJ

机构：

[1] Univ Tennessee, Dept Chem, Knoxville, TN 37996 USA

[2] Oak Ridge Natl Lab, Div Chem & Analyt Sci, Oak Ridge, TN 37831 USA

来源：

ANALYTICAL CHEMISTRY | 1999年 / 71卷 / 04期

关键词：

D O I：

10.1021/ac981259r

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Laser-induced fluorescence spectroscopy has been employed to probe the electrospray plume through measurement of the emission spectra of fluorescent dyes in the spray stream. The system is evaluated with respect to sensitivity and reproducibility using solutions of Eosin Y under typical electrospray conditions. Application of the technique for monitoring spray-induced pH changes is demonstrated using fluorescence signals from a pH-sensitive fluorophore, carboxyseminaphthorhodafluor-1 (C.SNARF-1). To our knowledge, this is the first direct measurement of pH within the electrospray plume.

引用

页码：769 / 776

页数：8

共 45 条

[1] DOES THE OBSERVATION OF NONCOVALENT COMPLEXES BETWEEN BIOMOLECULES BY ELECTROSPRAY-IONIZATION MASS-SPECTROMETRY NECESSARILY REFLECT SPECIFIC SOLUTION INTERACTIONS [J].