Electrokinetic supercharging for highly efficient peptide preconcentration in capillary zone electrophoresis

被引：47

作者：

Busnel, Jean-Marc ^{[1
]}

Lion, Niels ^{[1
]}

Girault, Hubert H. ^{[1
]}

机构：

[1] Ecole Polytech Fed Lausanne, SB ISIC LEPA, Lab Electrochim Phys & Analyt, CH-1015 Lausanne, Switzerland

来源：

ELECTROPHORESIS | 2008年 / 29卷 / 07期

关键词：

capillary zone electrophoresis; electrokinetic supercharging; stacking; transient isotachophoresis; tryptic digest;

D O I：

10.1002/elps.200700643

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Electrokinetic supercharging has been integrated in CZE for the development of a highly sensitive methodology for protein tryptic digest analysis. A careful choice of the experimental conditions led to sensitivity enhancement factors between 1000 and 10 000 whilst maintaining a satisfactory resolution. Peptides in the low nanomolar concentration range have been detected despite the use of the poorly sensitive UV absorbance detection mode. The buffer system used in this study is fully suitable for coupling CE to MS.

引用

页码：1565 / 1572

页数：8

共 31 条

[1] Selective enrichment and ultrasensitive identification of trace peptides in proteome analysis using transient capillary isotachophoresis/zone electrophoresis coupled with nano-ESI-MS [J].