IL-27 Modulates Chemokine Production in TNF-α-Stimulated Human Oral Epithelial Cells

Background/Aims: Interleukin-27 (IL-27) is a cytokine which belongs to the IL-12 family. However, the role of IL-27 in the pathogenesis of periodontal disease is uncertain. The aim of this study was to examine the effect of IL-27 on chemokine production in TNF-alpha-stimulated human oral epithelial cells (TR146). Methods: We measured chemokine production in TR146 by ELISA. We used western blot analysis to detect the phosphorylation levels of signal transduction molecules, including STAT1 and STAT3 in TR146. We used inhibitors to examine the role of STAT1 and STAT3 activation. Results: IL-27 increased CXCR3 ligands production in TNF-alpha-stimulated TR146. Meanwhile, IL-27 suppressed IL-8 and CCL20 production induced by TNF-alpha. STAT1 phosphorylation level in IL-27 and TNF-alpha-stimulated TR146 was enhanced in comparison to TNF-alpha-stimulated TR146. STAT3 phosphorylation level in IL-27-treated TR146 did not change by TNF-alpha. Both STAT1 inhibitor and STAT3 inhibitor decreased CXCR3 ligands production. STAT1 inhibitor overrode the inhibitory effect of IL-27 on IL-8 and CCL20 production in TNF-alpha-stimulated TR146. Meanwhile, STAT3 inhibitor did not modulate IL-8 and CCL20 production. Conclusion: IL-27 might control leukocyte migration in periodontal lesion by modulating chemokine production from epithelial cells. (C) 2017 The Author(s) Published by S. Karger AG, Basel