Diurnal variation in pH reduces digestion and synthesis of microbial protein when pasture is fermented in continuous culture

Many models of digestion assume steady-state conditions and do not account for diurnal variation in the rumen environment. This experiment examined the relationships between diurnal pH, pasture digestion, and microbial protein synthesis. Four dual-flow continuous culture fermenters were used to test the effect of increasing time at suboptimal pH on parameters of digestion. Fermentation of high quality pasture was controlled at pH 5.4 (suboptimal) for four intervals during each 24-h period (0, 4, 8, and 12 h) according to a 4 x 4 Latin square design. During the remainder of each day, pH was controlled at 6.3 (optimal). Samples were collected during the last 3 d of each of the four 9-d experimental periods. A negative quadratic relationship was observed between time at suboptimal pH and apparent digestibility of organic matter and dry matter. The largest reduction in digestibility of organic matter, dry matter, and neutral detergent fiber was exhibited after 4 h at suboptimal pH. A negative linear relationship was found between time at suboptimal pH and microbial N flow, with the greatest decline in microbial N flow occurring at 12 h at suboptimal pH. These results suggest that the period of time that pH is below optimal may be more critical for digestion than the relationship between mean daily pH and optimal pH. Modeling nonsteady-state ruminal conditions to account for diurnal variation in the ruminal environment may improve the prediction of digestion, especially fiber.