Seamless cloning and gene fusion

被引：95

作者：

Lu, Q ^{[1
]}

机构：

[1] GlaxoSmithKline, Gene Express & Prot Biochem, Discovery Res, King Of Prussia, PA 19406 USA

来源：

TRENDS IN BIOTECHNOLOGY | 2005年 / 23卷 / 04期

关键词：

D O I：

10.1016/j.tibtech.2005.02.008

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Gene fusion technology is a key tool in facilitating gene function studies. Hybrid molecules in which all the components are joined precisely, without the presence of intervening and unwanted extraneous sequences, enable accurate studies of molecules and the characterization of individual components. This article reviews situations in which seamlessly fused genes and proteins are required or desired and describes molecular approaches that are available for generating these hybrid molecules.

引用

页码：199 / 207

页数：9

共 70 条

[1] LIGATION-INDEPENDENT CLONING OF PCR PRODUCTS (LIC-PCR) [J].

ASLANIDIS, C ;

DEJONG, PJ .

NUCLEIC ACIDS RESEARCH, 1990, 18 (20) :6069-6074

[2] Design of highly specific cytotoxins by using trans-splicing ribozymes [J].

Ayre, BG ;

Köhler, U ;

Goodman, HM ;

Haseloff, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (07) :3507-3512

[3] THE DEGRADATION SIGNAL IN A SHORT-LIVED PROTEIN [J].

BACHMAIR, A ;

VARSHAVSKY, A .

CELL, 1989, 56 (06) :1019-1032

[4] INVIVO HALF-LIFE OF A PROTEIN IS A FUNCTION OF ITS AMINO-TERMINAL RESIDUE [J].

BACHMAIR, A ;

FINLEY, D ;

VARSHAVSKY, A .

SCIENCE, 1986, 234 (4773) :179-186

[5]

BAUER JC, 1998, Patent No. 6391548

[6]

BAUER JC, 1998, Patent No. 5789166

[7]

Braman J, 2002, IN VITRO MUTAGENESIS

[8] RAPID CLONING BY HOMOLOGOUS RECOMBINATION IN-VIVO [J].

BUBECK, P ;

WINKLER, M ;

BAUTSCH, W .

NUCLEIC ACIDS RESEARCH, 1993, 21 (15) :3601-3602

[9] PCR-based gene synthesis and protein NMR spectroscopy [J].

Casimiro, DR ;

Wright, PE ;

Dyson, HJ .

STRUCTURE, 1997, 5 (11) :1407-1412

[10] Universal restriction site-free cloning method using chimeric primers [J].

Chen, GJ ;

Qiu, N ;

Page, MGP .

BIOTECHNIQUES, 2002, 32 (03) :516-+

← 1 2 3 4 5 6 7 →