Structure-specific DNA cleavage by 5′ nucleases

被引：55

作者：

Ceska, TA

Sayers, JR

机构：

[1] Celltech Therapeut, Slough SL1 4EN, Berks, England

[2] Univ Sheffield, Royal Hallamshire Hosp, Div Med & Mol Genet, Sheffield S10 2JF, S Yorkshire, England

来源：

TRENDS IN BIOCHEMICAL SCIENCES | 1998年 / 23卷 / 09期

基金：

英国惠康基金;

关键词：

D O I：

10.1016/S0968-0004(98)01259-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Many processes, such as DNA replication, recombination and repair, produce branched DNA structures. These bifurcated molecules are trimmed by a group of homologous 5'-3' exonucleases (also known as 5' nucleases) in structure-specific cleavage reactions. X-ray crystallographical and biochemical studies suggest that ssDNA substrates become threaded through the 5'-3' exonucleases, where hydrolysis is effected with the aid of divalent metal cations.

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收藏

页码：331 / 336

页数：6

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