Switching transgene expression in the brain using an adenoviral tetracycline-regulatable system

被引：120

作者：

Harding, TC ^{[1
]}

Geddes, BJ ^{[1
]}

Murphy, D ^{[1
]}

Knight, D ^{[1
]}

Uney, JB ^{[1
]}

机构：

[1] Univ Bristol, Dept Med, Bristol BS2 8HW, Avon, England

来源：

NATURE BIOTECHNOLOGY | 1998年 / 16卷 / 06期

基金：

英国惠康基金; 英国生物技术与生命科学研究理事会;

关键词：

gene therapy; gene regulation; hippocampus;

D O I：

10.1038/nbt0698-553

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have developed a tetracycline-regulatable adenoviral transfection system that mediates efficient long-term transfer of genes into neuronal cells in vivo. This system allows gene expression to be switched on, then off, and back on again simply by administering or removing doxycycline from the animals' drinking water. This regulatable adenoviral vector system should be of value in behavioral studies and in vivo studies of neuronal gene function, and may further the development of effective gene therapy strategies in the brain.

引用

页码：553 / 555

页数：3

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