Chitotriosidase as a marker of macrophage stimulation

被引：24

作者：

Korolenko, TA

Zhanaeva, SY

Falameeva, OV

Kaledin, VI

Filyushina, EE

Buzueva, II

Paul', GA

机构：

[1] Russian Acad Med Sci, Inst Physiol, Inst Cell Biochem & Physiol, Moscow 109801, Russia

[2] Russian Acad Sci, Inst Cytol & Genet, Moscow 117901, Russia

[3] Reg Diagnost Ctr, Dept Med Genet, Novosibirsk, Russia

来源：

BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE | 2000年 / 130卷 / 10期

关键词：

macrophage stimulation; chitotriosidase; lysosomes; lysosomal storage diseases;

D O I：

10.1023/A:1002849420541

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Serum chitotriosidase activity was determined in different conditions accompanied by macrophage stimulation. Stimulation of macrophages with zymosan, yeast polysaccharide carboxymethylglucan (fraction II), and lysosomotropic preparation Triton WR-1339 1.5-2.0-fold increased enzyme activity. Chitotriosidase activity in intact Wistar rats was similar to that in humans, while in CBA and A/Sn mice this parameter was 5-fold higher. Sharp increase in chitotriosidase activity in the serum from patients with type I Gaucher's disease was probably related to intense secretion of the enzyme by macrophages. Under experimental conditions, stimulation of rat and mouse macrophages (mainly liver cells) caused no increase in chitotriosidase activity typical of patients with Gaucher's disease.

引用

页码：948 / 950

页数：3

共 10 条

[1] Strong induction of members of the chitinase family of proteins in atherosclerosis - Chitotriosidase and human cartilage gp-39 expressed in lesion macrophages [J].