Hydrogen peroxide biosensor based on sol-gel-derived glasses doped with Eastman AQ polymer

被引：42

作者：

Chen, X ^{[1
]}

Zhang, JZ ^{[1
]}

Wang, BQ ^{[1
]}

Cheng, GJ ^{[1
]}

Dong, SJ ^{[1
]}

机构：

[1] Chinese Acad Sci, Changchun Inst Appl Chem, Electroanalyt Chem Lab, Changchun 130022, Peoples R China

来源：

ANALYTICA CHIMICA ACTA | 2001年 / 434卷 / 02期

基金：

中国国家自然科学基金;

关键词：

sol-gel; Eastman AQ; amperometric biosensor; thionine; horseradish peroxidase;

D O I：

10.1016/S0003-2670(01)00830-3

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A hydrogen peroxide biosensor based on sol-gel-derived glasses doped with poly(ester sulfonic acid) Eastman AQ 55D was constructed. Thionine (TH), as a mediator, was incorporated in this matrix by electrostatic force between TH+ and the negatively charged sulfonic acid group in Eastman AQ polymer. Performance and characteristics of the sensor were evaluated with respect to response time, sensitivity and storage stability. The enzyme electrode has a sensitivity of 11.36 muA mM(-1) with a detection limit of 5.0 x 10(-7) M H2O2, and the sensor achieved 95% of the steady state current within 20 s. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：255 / 260

页数：6

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