Species-specific identification of human adenoviruses by a multiplex PCR assay

被引:220
作者
Xu, WH [1 ]
McDonough, MC [1 ]
Erdman, DD [1 ]
机构
[1] Ctr Dis Control & Prevent, Resp & Enter Viruses Branch, Div Viral & Rickettsial Dis, Atlanta, GA 30333 USA
关键词
D O I
10.1128/JCM.38.11.4114-4120.2000
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A multiplex PCR assay was developed by using primers to the fiber gene that could differentiate human adenovirus (Ad) species A through F in a single amplification reaction, The assay correctly identified the species of all 49 recognized Ad prototype strains as well as 180 geographically and temporally diverse Ad field isolates. Ad serotype 6 (Ad6) (species C), Ad16 (species B), Ad31 (species A), and Ad40 and Ad41 (species F) could also be distinguished by amplicon size within each respective species. In comparison, a previously described Ad species-specific multiplex PCR assay that used primers to the Ad hexon gene gave equivocal results with several serotypes of species B, whereas our multiplex assay amplified all species B serotypes equally well. Our multiplex PCR assay will permit rapid, accurate, and cost-effective classification of Ad isolates.
引用
收藏
页码:4114 / 4120
页数:7
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