Lipopolysaccharide induced expression of pentraxin 3 in human neutrophils and monocyte-derived macrophages

被引：43

作者：

Imamura, Masaru ^{[1
]}

Kawasaki, Takashi

Savchenko, Alexander S.

Ohashi, Riuko

Jiang, Shuying ^{[3
]}

Miyamoto, Kyoko ^{[3
]}

Ito, Yukio ^{[3
]}

Iwanari, Hiroko ^{[3
]}

Sagara, Mina ^{[3
]}

Tanaka, Toshlya ^{[4
]}

Hamakubo, Takao ^{[4
]}

Kodama, Tatsuhiko ^{[4
]}

Uchlyama, Makoto ^{[2
]}

Naito, Makoto ^{[1
]}

机构：

[1] Niigata Univ, Grad Sch Med & Dent Sci, Dept Cellular Funct, Div Cellular & Mol Pathol,Chuou Ku, Niigata 9518510, Japan

[2] Niigata Univ, Grad Sch Med & Dent Sci, Dept Homeostat Regulat & Dev, Div Pediat, Niigata 9518510, Japan

[3] Perseus Proteom Inc, Tokyo, Japan

[4] Univ Tokyo, Adv Sci & Technol Res Ctr, Lab Syst Biol & Med, Tokyo, Japan

来源：

CELLULAR IMMUNOLOGY | 2007年 / 248卷 / 02期

关键词：

pentraxin; 3; neutrophil; monocyte-derived macrophage; acute coronary syndrome;

D O I：

10.1016/j.cellimm.2007.09.003

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Pentraxin 3 (PTX3) is a prototype protein of long pentraxin. PTX3 is produced by various cells, such as monocytes/macrophages (M phi s) in response to lipopolysaccharide (LPS) and proinflammatory signals. We performed immunoblotting, immunohistochemical staininig. reverse transcriptase-polymerase chain reaction (RT-PCR), quantitative real-time PCR and enzyme-linked immunosorbent assay (ELISA) of PTX3 in human monocyte-derived M phi s and neutrophils. PTX3 expression was observed in the cytoplasm of both GM-CSF induced monocyte-derived M phi (GM-M phi) and M-CSF induced monocyte-derived M phi (M-M phi). PTX3 level in both M phi s was up-regulated at 24 It after LPS stimulation. Moreover, we confirmed PTX3 expression in freshly isolated neutrophils, and PTX3 level was distinctly up-regulated at 6 and 24 It after LPS stimulation. These findings suggested that PTX3 expression, not only in M phi s, but also in neutrophils, may reflect the role of PTX3 in inflammation. We believe that PTX3 can contribute as a diagnostic tool to evaluate inflammation at peripheral sites. (C) 2007 Elsevier Inc. All rights reserved.

引用

页码：86 / 94

页数：9

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