Identification of multiple chondroitin sulfate A (CSA)-binding domains in the var2CSA gene transcribed in CSA-binding parasites

被引：126

作者：

Gamain, B

Trimnell, AR

Scheidig, C

Scherf, A

Miller, LH

Smith, JD

机构：

[1] Inst Pasteur, URA 2581, Unite Biol Interact Hote Parasite, F-75724 Paris 15, France

[2] Seattle Biomed Res Inst, Seattle, WA 98109 USA

[3] Univ Washington, Dept Pathobiol, Seattle, WA 98195 USA

[4] NIAID, Lab Mol & Vector Res, NIH, Bethesda, MD 20892 USA

来源：

JOURNAL OF INFECTIOUS DISEASES | 2005年 / 191卷 / 06期

关键词：

D O I：

10.1086/428137

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Malaria in pregnancy is a serious complication associated with parasitized erythrocyte (PE) sequestration in the placenta. Recent work suggests that var genes could play an important role in PE binding to chondroitin sulfate A (CSA), a primary placental adherence receptor. Here, we confirm that var2CSA is transcriptionally up-regulated in CSA-binding parasites and define CSA-binding domains in var2CSA. The identification of multiple binding domains in var2CSA strengthens the evidence for their involvement in malaria during pregnancy and may have applications for the development of a vaccine against malaria in pregnancy.

引用

收藏

页码：1010 / 1013

页数：4

相关论文

共 15 条

[1] Recovery of adhesion to chondroitin-4-sulphate in Plasmodium falciparum varCSA disruption mutants by antigenically similar PfEMP1 variants [J].

Andrews, KT ;

Pirrit, LA ;

Przyborski, JM ;

Sanchez, CP ;

Sterkers, Y ;

Ricken, S ;

Wickert, H ;

Lépolard, C ;

Avril, M ;

Scherf, A ;

Gysin, R ;

Lanzer, M .

MOLECULAR MICROBIOLOGY, 2003, 49 (03) :655-669

[2] Adhesion of Plasmodium falciparum-infected erythrocytes to hyaluronic acid in placental malaria [J].

Beeson, JG ;

Rogerson, SJ ;

Cooke, BM ;

Reeder, JC ;

Chai, WG ;

Lawson, AM ;

Molyneux, ME ;

Brown, GV .

NATURE MEDICINE, 2000, 6 (01) :86-90

[3] The sick placenta -: The role of malaria [J].

Brabin, BJ ;

Romagosa, C ;

Abdelgalil, S ;

Menéndez, C ;

Verhoeff, FH ;

McGready, R ;

Fletcher, KA ;

Owens, S ;

d'Alessandro, U ;

Nosten, F ;

Fischer, PR ;

Ordi, J .

PLACENTA, 2004, 25 (05) :359-378

[4] Plasmodium falciparum domain mediating adhesion to chondroitin sulfate A:: A receptor for human placental infection [J].

Buffet, PA ;

Gamain, B ;

Scheidig, C ;

Baruch, D ;

Smith, JD ;

Hernandez-Rivas, R ;

Pouvelle, B ;

Oishi, S ;

Fujii, N ;

Fusai, T ;

Parzy, D ;

Miller, LH ;

Gysin, J ;

Scherf, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (22) :12743-12748

[5] Adherence of Plasmodium falciparum to chondroitin sulfate A in the human placenta [J].

Fried, M ;

Duffy, PE .

SCIENCE, 1996, 272 (5267) :1502-1504

[6] Maternal antibodies block malaria [J].

Fried, M ;

Nosten, F ;

Brockman, A ;

Brabin, BT ;

Duffy, PE .

NATURE, 1998, 395 (6705) :851-852

[7] Identification of a 67-amino-acid region of the Plasmodium falciparum variant surface antigen that binds chondroitin sulphate A and elicits antibodies reactive with the surface of placental isolates [J].

Gamain, B ;

Smith, JD ;

Avril, M ;

Baruch, DI ;

Scherf, A ;

Gysin, J ;

Miller, LH .

MOLECULAR MICROBIOLOGY, 2004, 53 (02) :445-455

[8] Molecular basis for the dichotomy in Plasmodium falciparum adhesion to CD36 and chondroitin sulfate A [J].

Gamain, B ;

Gratepanche, S ;

Miller, LH ;

Baruch, DI .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (15) :10020-10024

[9] Evidence for the importance of genetic structuring to the structural and functional specialization of the Plasmodium falciparum var gene family [J].

Kraemer, SM ;

Smith, JD .

MOLECULAR MICROBIOLOGY, 2003, 50 (05) :1527-1538

[10] A simple RNA analysis method shows var and rif multigene family expression patterns in Plasmodium falciparum [J].

Kyes, S ;

Pinches, R ;

Newbold, C .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 2000, 105 (02) :311-315