Polyhemoglobin superoxide dismutase catalase as a blood substitute with antioxidant properties

被引：165

作者：

D'Agnillo, F ^{[1
]}

Chang, TMS ^{[1
]}

机构：

[1] McGill Univ, Fac Med, Artificial Cells & Organs Res Ctr, Montreal, PQ, Canada

来源：

NATURE BIOTECHNOLOGY | 1998年 / 16卷 / 07期

关键词：

protein engineering; tissue engineering; hemoglobin;

D O I：

10.1038/nbt0798-667

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Polyhemoglobin-superoxide dismutase-catalase is designed to function as an oxygen carrier with antioxidant properties. This is based on cross-linking hemoglobin with superoxide dismutase and catalase (PolyHb-SOD-CAT). This study describes the structural and antioxidant properties of this solution. Our studies show that superoxide dismutase and catalase retain their enzymatic activity following glutaraldehyde polymerization with 8:1 and 16:1 glutaraldehyde:hemoglobin ratio. We have analyzed the optimal SOD/CAT ratios to prevent oxidation of hemoglobin in the presence of oxygen free radicals. The circulation half-life of crosslinked hemoglobin, SOD, and catalase in Sprague-Dawley rats correlates with the degree of polymerization as determined by high-performance molecular weight gel filtration. PolyHb-SOD-CAT decreases the formation of oxygen radicals compared with PolyHb in a rat intestinal ischemia-reperfusion model.

引用

页码：667 / 671

页数：5

共 36 条

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