Alternative splicing regulates the transcriptional activity of Drosophila heat shock transcription factor in response to heat/cold stress

被引：79

作者：

Fujikake, N

Nagai, Y

Popiel, HA

Kano, H

Yamaguchi, M

Toda, T

机构：

[1] Osaka Univ, Grad Sch Med, Dept Med Genet, Div Clin Genet, Suita, Osaka 5650871, Japan

[2] Kyoto Inst Technol, Fac Text Sci, Dept Appl Biol, Kyoto 6068585, Japan

来源：

FEBS LETTERS | 2005年 / 579卷 / 17期

关键词：

heat shock transcription factor; alternative splicing; heat stress; cold stress; Drosophila;

D O I：

10.1016/j.febslet.2005.05.074

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Heat shock transcription factor 1 (HSF1) mediates the induction of heat shock proteins in response to various types of stress. Although HSF1 activity is regulated by its post-translational modifications, alterations in mRNA expression have also been suggested. We here identified three new alternatively spliced isoforms of Drosophila HSF (dHSF) mRNA, named dHSFb, dHSFc, and dHSFd. We found that the ratio of dHSFb increases upon heat exposure, while that of dHSFd increases upon cold exposure. The dHSFc and dHSFd isoforms showed greater transcriptional activity than the other isoforms. Our findings suggest that alternative splicing regulates the transcriptional activity of dHSF. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：3842 / 3848

页数：7

共 26 条

[1] Heat- and cold-inducible regulation of HSP70 expression in zebrafish ZF4 cells [J].