Spontaneous cleavage of 4′-DNA radicals under aerobic conditions:: Apparent discrepancy between trapping rates and cleavage products

被引：39

作者：

Dussy, A ^{[1
]}

Meggers, E ^{[1
]}

Giese, B ^{[1
]}

机构：

[1] Univ Basel, Dept Chem, CH-4056 Basel, Switzerland

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 1998年 / 120卷 / 30期

关键词：

D O I：

10.1021/ja980036f

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The O-2-induced strand scission of 4'-DNA radicals is initiated by a reversible O-2 addition reaction. The rate coefficient of the O-2 release: from the single-stranded 4'-DNA peroxyl radical is 1.0 s(-1) at 20 degrees C. Because of this reversibility, an O-2-dependent strand cleavage occurs only in the presence of H-donors that trap the 4'-DNA peroxyl radicals, yielding DNA hydroperoxides. At very low H-donor concentrations the strand scission is the result of an O-2-independent, spontaneous reaction even under aerobic conditions. Nevertheless, oxidative products can de formed from the fragments of this spontaneous DNA cleavage.

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页码：7399 / 7403

页数：5

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