High level expression of functionally active human lactoferrin in silkworm larvae

被引:37
作者
Liu, T
Zhang, YZ [1 ]
Wu, XF
机构
[1] Zhejiang Sci Tech Univ, Inst Biochem, Hangzhou 310018, Peoples R China
[2] Zhejiang Univ, Coll Life Sci, Inst Biochem, Hangzhou 310029, Peoples R China
[3] Chinese Acad Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China
关键词
human lactoferrin; silkworm larvae; recombinant baculovirus; colitis;
D O I
10.1016/j.jbiotec.2005.05.005
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
In this paper, recombinant human lactoferrin (rhLf) was expressed very well using Bombyx mori nuclear polyhedrosis baculovirus expression system. Infection of silkworm larvae with recombinant virus, vBm-hLf, the rhLf was efficiently secreted into larvae hemolymph and the concentration of product purified was about 65 mu g/ml. The isolated rhLf molecular mass was similar to 78 kDa, lower than that of the human lactoferrin (hLf) standards, which may be due to incomplete glycosylation or protein degradation. Furthermore, the rhLf was characterized and its biological activities were evaluated by in vivo bioassay using dextran sodium sulfate (DSS)-induced colitis mouse model that mimics some characteristics of colitis disease in human. We conclude that silkworm expression system can be used successfully to express functional human lactoferrin. (C) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码:246 / 256
页数:11
相关论文
共 29 条
  • [1] Lactoferrin is responsible for the fungistatic effect of human milk
    Andersson, Y
    Lindquist, S
    Lagerqvist, C
    Hernell, O
    [J]. EARLY HUMAN DEVELOPMENT, 2000, 59 (02) : 95 - 105
  • [2] Mice with a selective deletion of the CC chemokine receptors 5 or 2 are protected from dextran sodium sulfate-mediated colitis:: Lack of CC chemokine receptor 5 expression results in a NK1.1+ lymphocyte-associated Th2-type immune response in the intestine
    Andres, PG
    Beck, PL
    Mizoguchi, E
    Mizoguchi, A
    Bhan, AK
    Dawson, T
    Kuziel, WA
    Maeda, N
    MacDermott, RP
    Podolsky, DK
    Reinecker, HC
    [J]. JOURNAL OF IMMUNOLOGY, 2000, 164 (12) : 6303 - 6312
  • [3] Ausubel FM., 1993, Current Protocols in Molecular Biology
  • [4] BEZAULT J, 1994, CANCER RES, V54, P2310
  • [5] COOPER HS, 1993, LAB INVEST, V69, P238
  • [6] Dieleman LA, 1998, CLIN EXP IMMUNOL, V114, P385
  • [7] EXPERIMENTAL-MODELS OF INFLAMMATORY BOWEL-DISEASE
    ELSON, CO
    SARTOR, RB
    TENNYSON, GS
    RIDDELL, RH
    [J]. GASTROENTEROLOGY, 1995, 109 (04) : 1344 - 1367
  • [8] Inflammatory bowel disease: Etiology and pathogenesis
    Fiocchi, C
    [J]. GASTROENTEROLOGY, 1998, 115 (01) : 182 - 205
  • [9] Anti-inflammatory activities of human lactoferrin in acute dextran sulphate-induced colitis in mice
    Håversen, LA
    Baltzer, L
    Dolphin, G
    Hanson, LÅ
    Mattsby-Baltzer, I
    [J]. SCANDINAVIAN JOURNAL OF IMMUNOLOGY, 2003, 57 (01) : 2 - 10
  • [10] HIDEFUMI K, 2001, J NUTR, V131, P2121