Temperature and growth-phase effects on Aeromonas hydrophila survival in natural seawater microcosms:: role of protein synthesis and nucleic acid content on viable but temporarily nonculturable response

被引:44
作者
Maalej, S
Denis, M
Dukan, S [1 ]
机构
[1] Fac Sci, Microbiol Lab, Sfax 3018, Tunisia
[2] Univ Mediterranee, CNRS, UMR 6535, Lab Oceanog & Biogeochim, F-13288 Marseille 9, France
[3] Univ Mediterranee, CNRS, UMR 6117, Lab Microbiol Marine, F-13288 Marseille 9, France
来源
MICROBIOLOGY-SGM | 2004年 / 150卷
关键词
D O I
10.1099/mic.0.26639-0
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The behaviour of Aeromonas hydrophila in nutrient-poor filter-sterilized seawater was investigated at 23 and 5 degreesC with respect to its growth phase. At both temperatures, the culturable A. hydrophila population declined below the detection level (0.1 c.f.u. ml(-1)) after 3-5 weeks, depending on the initial physiological state of the cells. During the first week, starved A. hydrophila cells appeared more resistant to the seawater stress at 5 degreesC than cells initially in the exponential growth phase. This difference was not observed at 23 degreesC, where de novo protein synthesis seemed to be required for long-term adaptation of cells from the exponential growth phase. Over the duration of the experiments, intact and total cell concentrations were not significantly affected, indicating that bacteria had entered a so-called viable but nonculturable state (VBNC). However, the incubated bacteria rapidly became heterogeneous with respect to their nucleic acid content, and their cell size decreased faster at 23 than at 5 degreesC. Resuscitation of VBNC cells was attempted by a temperature shift from 5 to 23 degreesC without exogenous nutrient addition. Comparison of the growth rates of the stressed population and of the untreated bacteria growing in the same autoclaved initial cell suspension showed significantly faster growth for the stressed cells, suggesting that in addition to growth of the few culturable stressed cells, a proportion of injured cells became culturable.
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页码:181 / 187
页数:7
相关论文
共 37 条
[1]  
Baumann P., 1984, Bergey's Manual of systematic bacteriology. Volume 1, P516
[2]   Recovery of hydrogen peroxide-sensitive culturable cells of Vibrio vulnificus gives the appearance of resuscitation from a viable but nonculturable state [J].
Bogosian, G ;
Aardema, ND ;
Bourneuf, EV ;
Morris, PJL ;
O'Neil, JP .
JOURNAL OF BACTERIOLOGY, 2000, 182 (18) :5070-5075
[3]   A matter of bacterial life and death [J].
Bogosian, G ;
Bourneuf, EV .
EMBO REPORTS, 2001, 2 (09) :770-774
[4]  
Colwell RR, 2000, NONCULTURABLE MICROORGANISMS IN THE ENVIRONMENT, P325
[5]   Differential oxidative damage and expression of stress defence regulons in culturable and non-culturable Escherichia coli cells [J].
Desnues, B ;
Cuny, C ;
Grégori, G ;
Dukan, S ;
Aguilaniu, H ;
Nyström, T .
EMBO REPORTS, 2003, 4 (04) :400-404
[6]   Recovery of culturability of an HOCl-stressed population of Escherichia coli after incubation in phosphate buffer: Resuscitation or regrowth? [J].
Dukan, S ;
Levi, Y ;
Touati, D .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1997, 63 (11) :4204-4209
[7]   Hypochlorous acid stress in Escherichia coli: Resistance, DNA damage, and comparison with hydrogen peroxide stress [J].
Dukan, S ;
Touati, D .
JOURNAL OF BACTERIOLOGY, 1996, 178 (21) :6145-6150
[8]   Oxidative stress defense and deterioration of growth-arrested Escherichia coli cells [J].
Dukan, S ;
Nyström, T .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (37) :26027-26032
[9]  
Gasol JM, 1999, APPL ENVIRON MICROB, V65, P4475
[10]   Incidence of mesophilic Aeromonas within a public drinking water supply in north-east Scotland [J].
Gavriel, AA ;
Landre, JPB ;
Lamb, AJ .
JOURNAL OF APPLIED MICROBIOLOGY, 1998, 84 (03) :383-392