Counting basic sites in oligopeptides via gas-phase ion chemistry

被引：50

作者：

Stephenson, JL ^{[1
]}

McLuckey, SA ^{[1
]}

机构：

[1] OAK RIDGE NATL LAB,DIV CHEM & ANALYT SCI,OAK RIDGE,TN 37831

来源：

ANALYTICAL CHEMISTRY | 1997年 / 69卷 / 03期

关键词：

D O I：

10.1021/ac961119m

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Cations derived from oligopeptides ranging from laminin fragment (5 residues) to beta-lactoglobulin (162 residues) have been subjected to gas-phase ion/molecule reactions with hydroiodic acid. The sum of the ion charge state and the maximum number of molecules of hydroiodic acid that attach to the ion is equal to the total number of lysines, arginines, histidines, and N-termini consisting of a primary amine for ions derived from all 21 oligopeptides studied, These results suggest that ion/molecule reactions can provide useful information regarding oligopeptide basic site number, which might be used as a criterion for searching protein data bases instead of, or in conjunction with, use of proteolytic digestion or gas-phase ion dissociation procedures.

引用

页码：281 / 285

页数：5

共 32 条

[1] MASS-SPECTROMETRIC DETERMINATION OF THE AMINO-ACID-SEQUENCE OF PEPTIDES AND PROTEINS [J].

BIEMANN, K ;

MARTIN, SA .

MASS SPECTROMETRY REVIEWS, 1987, 6 (01) :1-75

[2] INFLUENCE OF CYSTEINE TO CYSTEIC ACID OXIDATION ON THE COLLISION-ACTIVATED DECOMPOSITION OF PROTONATED PEPTIDES - EVIDENCE FOR INTRAIONIC INTERACTIONS [J].

BURLET, O ;

YANG, CY ;

GASKELL, SJ .

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 1992, 3 (04) :337-344

[3] Deuterium exchange reactions as a probe of biomolecule structure. Fundamental studies of cas phase H/D exchange reactions of protonated glycine oligomers with D2O, CD3OD, CD3CO2D, and ND3 [J].

Campbell, S ;

Rodgers, MT ;

Marzluff, EM ;

Beauchamp, JL .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1995, 117 (51) :12840-12854

[4] DEPROTONATION REACTIONS OF MULTIPLY PROTONATED UBIQUITIN IONS [J].

CASSADY, CJ ;

WRONKA, J ;

KRUPPA, GH ;

LAUKIEN, FH .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 1994, 8 (05) :394-400

[5] Role of the site of protonation in the low-energy decompositions of gas-phase peptide ions [J].

Cox, KA ;

Gaskell, SJ ;

Morris, M ;

Whiting, A .

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 1996, 7 (06) :522-531

[6] ELECTROSPRAY IONIZATION FOR MASS-SPECTROMETRY OF LARGE BIOMOLECULES [J].

FENN, JB ;

MANN, M ;

MENG, CK ;

WONG, SF ;

WHITEHOUSE, CM .

SCIENCE, 1989, 246 (4926) :64-71

[7] IDENTIFYING PROTEINS FROM 2-DIMENSIONAL GELS BY MOLECULAR MASS SEARCHING OF PEPTIDE-FRAGMENTS IN PROTEIN-SEQUENCE DATABASES [J].

HENZEL, WJ ;

BILLECI, TM ;

STULTS, JT ;

WONG, SC ;

GRIMLEY, C ;

WATANABE, C .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (11) :5011-5015

[8] Product ion charge state determination via ion/ion proton transfer reactions [J].

Herron, WJ ;

Goeringer, DE ;

McLuckey, SA .

ANALYTICAL CHEMISTRY, 1996, 68 (02) :257-262

[9] PROTEIN SEQUENCING BY TANDEM MASS-SPECTROMETRY [J].

HUNT, DF ;

YATES, JR ;

SHABANOWITZ, J ;

WINSTON, S ;

HAUER, CR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (17) :6233-6237

[10] PROTON-TRANSFER REACTIONS OF MASS-SELECTED MULTIPLY-CHARGED IONS [J].

HUNTER, AP ;

SEVERS, JC ;

HARRIS, FM ;

GAMES, DE .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 1994, 8 (05) :417-422

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