MicroRNA-194 inhibits epithelial to mesenchymal transition of endometrial cancer cells by targeting oncogene BMI-1

被引：201

作者：

Dong, Peixin ^{[1
,2
]}

Kaneuchi, Masanori ^{[1
,2
]}

Watari, Hidemichi ^{[1
,2
]}

Hamada, Junichi ^{[3
]}

Sudo, Satoko ^{[1
,2
]}

Ju, Jingfang ^{[4
]}

Sakuragi, Noriaki ^{[1
,2
]}

机构：

[1] Hokkaido Univ, Grad Sch Med, Dept Gynecol, Sapporo, Hokkaido, Japan

[2] Hokkaido Univ, Sch Med, Sapporo, Hokkaido 060, Japan

[3] Hokkaido Univ, Inst Med Genet, Div Canc Related Genes, Sapporo, Hokkaido, Japan

[4] SUNY Stony Brook, Dept Pathol, Med Ctr, Stony Brook, NY USA

来源：

MOLECULAR CANCER | 2011年 / 10卷

基金：

日本学术振兴会;

关键词：

ELEVATED EXPRESSION; GENE-EXPRESSION; PROTEIN BMI-1; METASTASIS; OVARIAN; BREAST; OVEREXPRESSION; SIGNATURE; INVASION; MUTATION;

D O I：

10.1186/1476-4598-10-99

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Background: Epithelial-mesenchymal transition (EMT) is the key process driving cancer metastasis. Oncogene/self renewal factor BMI-1 has been shown to induce EMT in cancer cells. Recent studies have implied that noncoding microRNAs (miRNAs) act as crucial modulators for EMT. The aims of this study was to determine the roles of BMI-1 in inducing EMT of endometrial cancer (EC) cells and the possible role of miRNA in controlling BMI-1 expression. Methods and results: We evaluated the expression of BMI-1 gene in a panel of EC cell lines, and detected a strong association with invasive capability. Stable silencing of BMI-1 in invasive mesenchymal-type EC cells up-regulated the epithelial marker E-cadherin, down-regulated mesenchymal marker Vimentin, and significantly reduced cell invasion in vitro. Furthermore, we discovered that the expression of BMI-1 was suppressed by miR-194 via direct binding to the BMI-1 3'-untranslated region 3'-UTR). Ectopic expression of miR-194 in EC cells induced a mesenchymal to epithelial transition (MET) by restoring E-cadherin, reducing Vimentin expression, and inhibiting cell invasion in vitro. Moreover, BMI-1 knockdown inhibited in vitro EC cell proliferation and clone growth, correlated with either increased p16 expression or decreased expression of stem cell and chemoresistance markers (SOX-2, KLF4 and MRP-1). Conclusion: These findings demonstrate the novel mechanism for BMI-1 in contributing to EC cell invasion and that repression of BMI-1 by miR-194 could have a therapeutic potential to suppress EC metastasis.

引用

页数：9

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