Isolation and characterization of biologically active lectin from Korean mistletoe, Viscum album var. Coloratum

被引：24

作者：

Lee, HS ^{[1
]}

Kim, YS

Kim, SB

Choi, BE

Woo, BH

Lee, KC

机构：

[1] Wonkwang Univ, Coll Pharm, Iksan 570749, South Korea

[2] Sungkyunkwan Univ, Coll Pharm, Drug Targeting Lab, Suwon 440746, South Korea

来源：

CELLULAR AND MOLECULAR LIFE SCIENCES | 1999年 / 55卷 / 04期

关键词：

Korean mistletoe; mistletoe lectin II (ML II); concanavalin A chromatography; MALDI-TOF;

D O I：

10.1007/s000180050324

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A mistletoe lectin was isolated from water extracts of Korean mistletoe, a subspecies of Viscum album, grown on Quercus mongolica using CM-Sepharose chromatography followed by an affinity chromatography on a concanavalin A-Sepharose column. The compound proved to be a mistletoe lectin II with D-galactose and N-acetyl-D-galactosamine specificity. Matrix-assisted laser desorption time-of-flight mass spectroscopy showed it to have an average molecular mass of 62.7 kDa and to consist of two subunits of 30.6 kDa and 32.5 kDa. it was a basic protein with isoelectric points of 9.4 and 9.6 by capillary isoelectric focusing and was cytotoxic to Molt4 cell.

引用

页码：679 / 682

页数：4

共 23 条

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