Identification of a malate chemoreceptor in Pseudomonas aeruginosa by screening for chemotaxis defects in an energy taxis-deficient mutant

被引：55

作者：

Alvarez-Ortega, Carolina

Harwood, Caroline S.

机构：

[1] Univ Washington, Dept Microbiol, Seattle, WA 98195 USA

[2] Univ Iowa, Dept Microbiol, Iowa City, IA 52242 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2007年 / 73卷 / 23期

关键词：

D O I：

10.1128/AEM.01898-07

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We found that a robust energy taxis response mediated by the Aer receptor can sometimes mask chemotaxis mediated by other methyl-accepting chemotaxis proteins (MCPs) in Pseudomonas aeruginosa. We identified PA2652 as a chemoreceptor for malate by screening aer mcp double mutants by using swarm plate assays.

引用

收藏

页码：7793 / 7795

页数：3

相关论文

共 29 条

[1] METHOD FOR MEASURING CHEMOTAXIS AND USE OF METHOD TO DETERMINE OPTIMUM CONDITIONS FOR CHEMOTAXIS BY ESCHERICHIA-COLI [J].

ADLER, J .

JOURNAL OF GENERAL MICROBIOLOGY, 1973, 74 (JAN) :77-91

[2] Evolutionary genomics reveals conserved structural determinants of signaling and adaptation in microbial chemoreceptors [J].

Alexander, Roger P. ;

Zhulin, Igor B. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (08) :2885-2890

[3] Energy taxis is the dominant behavior in Azospirillum brasilense [J].

Alexandre, G ;

Greer, SE ;

Zhulin, IB .

JOURNAL OF BACTERIOLOGY, 2000, 182 (21) :6042-6048

[4] Ecological role of energy taxis in microorganisms [J].

Alexandre, G ;

Greer-Phillips, S ;

Zhulin, IB .

FEMS MICROBIOLOGY REVIEWS, 2004, 28 (01) :113-126

[5]

Alvarez C., 2007, THESIS U IOWA IOWA C

[6] A signal transducer for aerotaxis in Escherichia coli [J].

Bibikov, SI ;

Biran, R ;

Rudd, KE ;

Parkinson, JS .

JOURNAL OF BACTERIOLOGY, 1997, 179 (12) :4075-4079

[7] Cluster II che genes from Pseudomonas aeruginosa are required for an optimal chemotactic response [J].

Ferrández, A ;

Hawkins, AC ;

Summerfield, DT ;

Harwood, CS .

JOURNAL OF BACTERIOLOGY, 2002, 184 (16) :4374-4383

[8] Aer and Tsr guide Escherichia coli in spatial gradients of oxidizable substrates [J].

Greer-Phillips, SE ;

Alexandre, G ;

Taylor, BL ;

Zhulin, IB .

MICROBIOLOGY-SGM, 2003, 149 :2661-2667

[9] Two different Pseudomonas aeruginosa chemosensory signal transduction complexes localize to cell poles and form and remould in stationary phase [J].

Guvener, Zehra Tuzun ;

Tifrea, Delia F. ;

Harwood, Caroline S. .

MOLECULAR MICROBIOLOGY, 2006, 61 (01) :106-118

[10] AROMATIC-ACIDS ARE CHEMOATTRACTANTS FOR PSEUDOMONAS-PUTIDA [J].

HARWOOD, CS ;

RIVELLI, M ;

ORNSTON, LN .

JOURNAL OF BACTERIOLOGY, 1984, 160 (02) :622-628