Epidermal CD8+ T cells in chronic plaque psoriasis are Tcl cells producing heterogeneous levels of interferon-gamma

被引:21
作者
Ovigne, JM [1 ]
Baker, BS [1 ]
Brown, DW [1 ]
Powles, AV [1 ]
Fry, L [1 ]
机构
[1] Univ London Imperial Coll Sci Technol & Med, London W2 1PG, England
关键词
CD8(+); T cells; chronic plaque psoriasis; interferon-gamma;
D O I
10.1034/j.1600-0625.2001.010003168.x
中图分类号
R75 [皮肤病学与性病学];
学科分类号
100206 ;
摘要
The majority of epidermal CD8(+) T cells in chronic plaque psoriasis are activated Tcl cells producing interferon-gamma and no interleukin-4, a small proportion of which express NK-T receptors. To quantitate their level of cytokine production and characterize them further, CD8(+) T cells were isolated from epidermal cell suspensions of lesional biopsies from 24 patients with chronic plaque psoriasis. T-cell lines (TCL) were established by culture of CD8(+) T cells with feeders and IL-2 for 11 days and expansion with PHA. Ten TCL were stained for surface markers; 6 were cloned with PHA by limiting dilution. Interferon-gamma, interleukin-4 and interleukin-10 production was measured by ELISA after PMA/anti-CD3 activation of 15 TCL and 39 CD8(+) T-cell clones. The 10 TCL stained were CD8 alpha beta (+) (93.3%), T-cell receptor-alpha beta (+) (99.5%), costimulatory molecule CD28(+) (90.1%), with a small CD8 alpha alpha (+) population (2.3%). No NK-T-cell receptor CD158a or CD158b expression was detected, whilst CD94 was expressed on 6.2% of cells in 6/9 TCL. All the TCL and 37/39 CD8(+) T-cell clones produced interferon-gamma but no or minimal interleukin-4 or interleukin-10. The TCL produced a wide range of interferon-gamma levels (138 to 15,020 pg/ml). Clones from 3 patients showed low levels (60 to 1,410 pg/ml), from 2 patients high levels (6,105 to 43,040 pg/ ml) and from 1 patient a wide range (405 to 36,010 pg/ml) of interferon-gamma production. Thus epidermal CD8(+) Tcl cells in chronic plaque psoriasis produce highly heterogeneous levels of interferon-gamma, which may reflect clinical diversity.
引用
收藏
页码:168 / 174
页数:7
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