Nematode-specific PCR primers for the 18S small subunit rRNA gene

被引：263

作者：

Floyd, RM

Rogers, AD

Lambshead, PJD

Smith, CR

机构：

[1] British Antarctic Survey, NERC, Cambridge CB3 0ET, England

[2] Nat Hist Museum, Dept Zool, London SW7 5BD, England

[3] Univ Hawaii Manoa, Dept Oceanog, Honolulu, HI 96822 USA

来源：

MOLECULAR ECOLOGY NOTES | 2005年 / 5卷 / 03期

关键词：

nematodes; 185 ribosomal RNA (SSU); specific amplification; DNA barcoding;

D O I：

10.1111/j.1471-8286.2005.01009.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A set of polymerase chain reaction primers were designed, which amplify a c.1 kb fragment of the 18S ribosomal DNA gene, and are specific to the phylum Nematoda. These have proven useful in isolating nematode genes from samples mixed with other biological material, particularly with application to DNA barcoding. Optimal reaction conditions are described. These primers have successfully amplified the correct fragment from a wide phylogenetic range of nematodes, and have so far generated no sequences from any other organismal group.

引用

页码：611 / 612

页数：2

共 5 条

[1] BASIC LOCAL ALIGNMENT SEARCH TOOL [J].