A Novel Assay for Quantifying the Number of Plasmids Encapsulated by Polymer Nanoparticles

被引：54

作者：

Bhise, Nupura S. ^{[1
]}

Shmueli, Ron B. ^{[1
]}

Gonzalez, Jose ^{[1
]}

Green, Jordan J. ^{[1
]}

机构：

[1] Johns Hopkins Univ, Sch Med, Dept Biomed Engn, Baltimore, MD 21231 USA

来源：

SMALL | 2012年 / 8卷 / 03期

关键词：

polymers; nanoparticles; nanoparticle tracking; gene expression; regenerative medicine; GENE-THERAPY; STEM-CELLS; DNA; SIZE; COMPLEXES; CATIONS; LENGTH;

D O I：

10.1002/smll.201101718

中图分类号：

O6 [化学];

学科分类号：

070301 [无机化学];

摘要：

Polymeric nanoparticles are promising for gene therapy and stem cell reprogramming using nonviral vectors. A novel assay utilizing nanoparticle tracking analysis is developed to easily quantify the number of plasmids within polymeric nanoparticles while in aqueous solution. Particles effective at co-transfecting primary human fibroblasts are approximately 100 nm in diameter and contain around 100 plasmids per particle. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：367 / 373

页数：7

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