Anaerobic redox cycling of iron by freshwater sediment microorganisms

被引:262
作者
Weber, KA
Urrutia, MM
Churchill, PF
Kukkadapu, RK
Roden, EE [1 ]
机构
[1] Univ Alabama, Dept Biol Sci, Tuscaloosa, AL 35487 USA
[2] Pacific NW Natl Lab, Richland, WA 99352 USA
关键词
D O I
10.1111/j.1462-2920.2005.00873.x
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The potential for microbially mediated anaerobic redox cycling of iron (Fe) was examined in a first-generation enrichment culture of freshwater wetland sediment microorganisms. Most probable number enumerations revealed the presence of significant populations of Fe(III)-reducing (approximately 10(8) cells ml(-1)) and Fe(II)-oxidizing, nitrate-reducing organisms (approximately 10(5) cells ml(-1)) in the freshwater sediment used to inoculate the enrichment cultures. Nitrate reduction commenced immediately following inoculation of acetate-containing (approximately 1 mM) medium with a small quantity (1% v/v) of wetland sediment, and resulted in the transient accumulation of NO2- and production of a mixture of gaseous end-products (N2O and N-2) and NH4+. Fe(III) oxide (high surface area goethite) reduction took place after NO3- was depleted and continued until all the acetate was utilized. Addition of NO3- after Fe(III) reduction ceased resulted in the immediate oxidation of Fe(II) coupled to reduction of NO3- to NH4+. No significant NO2- accumulation was observed during nitrate-dependent Fe(II) oxidation. No Fe(II) oxidation occurred in pasteurized controls. Microbial community structure in the enrichment was monitored by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified 16S rDNA and reverse transcription polymerase chain reaction-amplified 16S rRNA, as well as by construction of 16S rDNA clone libraries for four different time points during the experiment. Strong similarities in dominant members of the microbial community were observed in the Fe(III) reduction and nitrate-dependent Fe(II) oxidation phases of the experiment, specifically the common presence of organisms closely related (>= 95% sequence similarity) to the genera Geobacter and Dechloromonas. These results indicate that the wetland sediments contained organisms such as Geobacter sp. which are capable of both dissimilatory Fe(III) reduction and oxidation of Fe(II) with reduction of NO3- to NH4+. Our findings suggest that microbially catalysed nitrate-dependent Fe(II) oxidation has the potential to contribute to a dynamic anaerobic Fe redox cycle in freshwater sediments.
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收藏
页码:100 / 113
页数:14
相关论文
共 75 条
[1]   COMPETITION FOR ELECTRON-DONORS AMONG NITRATE REDUCERS, FERRIC IRON REDUCERS, SULFATE REDUCERS, AND METHANOGENS IN ANOXIC PADDY SOIL [J].
ACHTNICH, C ;
BAK, F ;
CONRAD, R .
BIOLOGY AND FERTILITY OF SOILS, 1995, 19 (01) :65-72
[2]   Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].
Altschul, SF ;
Madden, TL ;
Schaffer, AA ;
Zhang, JH ;
Zhang, Z ;
Miller, W ;
Lipman, DJ .
NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402
[3]   Anaerobic and aerobic oxidation of ferrous iron at neutral pH by chemoheterotrophic nitrate-reducing bacteria [J].
Benz, M ;
Brune, A ;
Schink, B .
ARCHIVES OF MICROBIOLOGY, 1998, 169 (02) :159-165
[4]   SULFOLOBUS - NEW GENUS OF SULFUR-OXIDIZING BACTERIA LIVING AT LOW PH AND HIGH-TEMPERATURE [J].
BROCK, TD ;
BROCK, KM ;
BELLY, RT ;
WEISS, RL .
ARCHIV FUR MIKROBIOLOGIE, 1972, 84 (01) :54-&
[5]   Reduction of (per)chlorate by a novel organism isolated from paper mill waste [J].
Bruce, RA ;
Achenbach, LA ;
Coates, JD .
ENVIRONMENTAL MICROBIOLOGY, 1999, 1 (04) :319-329
[6]   Competitive PCR-DGGE analysis of bacterial mixtures an internal standard and an appraisal of template enumeration accuracy [J].
Brüggemann, J ;
Stephen, JR ;
Chang, YJ ;
Macnaughton, SJ ;
Kowalchuk, GA ;
Kline, E ;
White, DC .
JOURNAL OF MICROBIOLOGICAL METHODS, 2000, 40 (02) :111-123
[7]   Screening for genetic diversity of isolates of anaerobic Fe(II)-oxidizing bacteria using DGGE and whole-cell hybridization [J].
BuchholzCleven, BEE ;
Rattunde, B ;
Straub, KL .
SYSTEMATIC AND APPLIED MICROBIOLOGY, 1997, 20 (02) :301-309
[8]   Microbial metabolism of benzene and the oxidation of ferrous iron under anaerobic conditions: Implications for bioremediation [J].
Caldwell, ME ;
Tanner, RS ;
Suflita, JM .
ANAEROBE, 1999, 5 (06) :595-603
[9]  
Chapelle F., 2001, GROUND WATER MICROBI
[10]   Biogenic magnetite formation through anaerobic biooxidation of Fe(II) [J].
Chaudhuri, SK ;
Lack, JG ;
Coates, JD .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2001, 67 (06) :2844-2848