Pathotyping Escherichia coli by using miniaturized DNA microarrays

被引:54
作者
Anjum, Muna F. [1 ]
Mafura, Muriel
Slickers, Peter
Ballmer, Karin
Kuhnert, Peter
Woodward, Martin J.
Ehricht, Ralf
机构
[1] Vet Labs Agcy, Dept Food & Environm Safety, Weybridge KT15 3NB, Surrey, England
[2] CLONDIAG Chip Technol GmbH, Jena, Germany
[3] Univ Bern, Vetsuisse Fac, Inst Vet Bacteriol, Bern, Switzerland
关键词
D O I
10.1128/AEM.00419-07
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The detection of virulence determinants harbored by pathogenic Escherichia coli is important for establishing the pathotype responsible for infection. A sensitive and specific miniaturized virulence microarray containing 60 oligonucleotide probes was developed. It detected six E. coli pathotypes and will be suitable in the future for high-throughput use.
引用
收藏
页码:5692 / 5697
页数:6
相关论文
共 25 条
[1]   Fast DNA serotyping of Escherichia coli by use of an oligonucleotide microarray [J].
Ballmer, Karin ;
Korczak, Lozena M. ;
Kuhnert, Peter ;
Slickers, Peter ;
Ehricht, Ralf ;
Haechler, Herbert .
JOURNAL OF CLINICAL MICROBIOLOGY, 2007, 45 (02) :370-379
[2]  
BRAUNT G, 2006, APPL ENVIRON MICROB, V72, P3780
[3]   Detecting and genotyping Escherichia coli O157:H7 using multiplexed PCR and nucleic acid microarrays [J].
Call, DR ;
Brockman, FJ ;
Chandler, DP .
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2001, 67 (1-2) :71-80
[4]   A DNA microarray for identification of virulence and antimicrobial resistance genes in Salmonella serovars and Escherichia coli [J].
Chen, S ;
Zhao, SH ;
McDermott, PF ;
Schroeder, CM ;
White, DG ;
Meng, JH .
MOLECULAR AND CELLULAR PROBES, 2005, 19 (03) :195-201
[5]   Microarray analysis of microbial virulence factors [J].
Chizhikov, V ;
Rasooly, A ;
Chumakov, K ;
Levy, DD .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2001, 67 (07) :3258-3263
[6]   Characterization of nonenterotoxigenic Escherichia coli strains producing F17 fimbriae isolated from diarrheic lambs and goat kids [J].
Cid, D ;
Sanz, R ;
Marín, I ;
De Greve, H ;
Ruiz-Santa-Quiteria, JA ;
Amils, R ;
De la Fuente, R .
JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (05) :1370-1375
[7]   PCR for detection of cdt-III and the relative frequencies of cytolethal distending toxin variant-producing Escherichia coli isolates from humans and cattle [J].
Clark, CG ;
Johnson, ST ;
Easy, RH ;
Campbell, JL ;
Rodgers, FG .
JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (07) :2671-2674
[8]   EVIDENCE FOR 2 TYPES OF CYTOTOXIC NECROTIZING FACTOR IN HUMAN AND ANIMAL CLINICAL ISOLATES OF ESCHERICHIA-COLI [J].
DERYCKE, J ;
GONZALEZ, EA ;
BLANCO, J ;
OSWALD, E ;
BLANCO, M ;
BOIVIN, R .
JOURNAL OF CLINICAL MICROBIOLOGY, 1990, 28 (04) :694-699
[9]   Use of a microarray to assess the distribution of plasmid and chromosomal virulence genes in strains of enteroaggregative Escherichia coli [J].
Jenkins, C ;
van Ijperen, C ;
Dudley, EG ;
Chart, H ;
Willshaw, GA ;
Cheasty, T ;
Smith, HR ;
Nataro, JP .
FEMS MICROBIOLOGY LETTERS, 2005, 253 (01) :119-124
[10]   Pathogenic Escherichia coli [J].
Kaper, JB ;
Nataro, JP ;
Mobley, HLT .
NATURE REVIEWS MICROBIOLOGY, 2004, 2 (02) :123-140