A global double-fluorescent cre reporter mouse

被引:2601
作者
Muzumdar, Mandar Deepak
Tasic, Bosiljka
Miyamichi, Kazunari
Li, Ling
Luo, Liqun [1 ]
机构
[1] Stanford Univ, Howard Hughes Med Inst, Dept Biol Sci, Stanford, CA 94305 USA
[2] Stanford Univ, Sch Med, Stanford, CA 94305 USA
关键词
mosaic; mT/mG; GPP; tdTomato; marker; membrane-targeted; recombination; cell morphology; lineage tracing;
D O I
10.1002/dvg.20335
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The Cre/loxP system has been used extensively for conditional mutagenesis in mice. Reporters of Cre activity are important for defining the spatial and temporal extent of Cre-mediated recombination. Here we describe mT/mG, a double-fluorescent Cre reporter mouse that expresses membrane-targeted tandem dimer Tomato (mT) prior to Cre-mediated excision and membrane-targeted green fluorescent protein (mG) after excision. We show that reporter expression is nearly ubiquitous, allowing visualization of fluorescent markers in live and fixed samples of all tissues examined. We further demonstrate that mG labeling is Cre-dependent, complementary to mT at single cell resolution, and distinguishable by fluorescence-activated cell sorting. Both membrane-targeted markers outline cell morphology, highlight membrane structures, and permit visualization of fine cellular processes. In addition to serving as a global Cre reporter, the mT/mG mouse may also be used as a tool for lineage tracing, transplantation studies, and analysis of cell morphology in vivo.
引用
收藏
页码:593 / 605
页数:13
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