Characterization and in vivo evaluation of chitosan-hydroxyapatite bone scaffolds made by one step coprecipitation method

被引：60

作者：

Danilchenko, Sergei N. ^{[1
]}

Kalinkevich, Oksana V. ^{[1
]}

Pogorelov, Maksim V. ^{[2
]}

Kalinkevich, Aleksei N. ^{[1
]}

Sklyar, Anatoly M. ^{[3
]}

Kalinichenko, Tatyana G. ^{[1
]}

Ilyashenko, Vyacheslav Y. ^{[1
]}

Starikov, Vadim V. ^{[4
]}

Bumeyster, Valentina I. ^{[2
]}

Sikora, Vitaly Z. ^{[2
]}

Sukhodub, Leonid F. ^{[1
]}

机构：

[1] Natl Acad Sci Ukraine, Dept Biophys, Inst Appl Phys, Sumy, Ukraine

[2] Sumy State Univ, Inst Med, Dept Human Anat, Sumy, Ukraine

[3] Sumy State Pedag Univ, Dept Chem, Sumy, Ukraine

[4] Natl Tech Univ, Kharkov Polytech Inst, Dept Met Phys, Kharkov, Ukraine

来源：

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A | 2011年 / 96A卷 / 04期

关键词：

chitosan; hydroxyapatite; scaffolds; biodegradation; osteogenesis; CALCIUM-PHOSPHATE; COMPOSITE SCAFFOLDS; TISSUE; SIZE; APATITE; CHITIN; MINERALIZATION; MEMBRANES; MATRICES; NETWORK;

D O I：

10.1002/jbm.a.33017

中图分类号：

R318 [生物医学工程];

学科分类号：

100103 [病原生物学];

摘要：

Chitosan/hydroxyapatite scaffolds could be used for bone regeneration in case the application of auto-or allografts is impossible. The objective of the present work was to characterize and study in vivo biodegradation of simple chitosan/hydroxyapatite scaffolds. For this purpose, a series of chitosan/hydroxyapatite composites has been synthesized in aqueous medium from chitosan solution and soluble precursor salts by a one step coprecipitation method. A study of in vivo behavior of the materials was then performed using model linear rats. Cylindrical-shaped rods made of the chitosan/hydroxyapatite composite material were implanted into tibial bones of the rats. After 5, 10, 15, and 24 days of implantation, histological and histomorpho-metric analyses of decalcified specimens were performed to evaluate the stages of biodegradation processes. Calcified specimens were examined by scanning electron microscopy with X-ray microanalysis to compare elemental composition and morphological characteristics of the implant and the bone during integration. Porous chitosan/hydroxyapatite scaffolds have shown osteoconductive properties and have been replaced in the in vivo experiments by newly formed bone tissue. (C) 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 96A: 639-647, 2011.

引用

页码：639 / 647

页数：9

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