Rabbit monoclonal Fab derived from a phage display library

被引：17

作者：

Foti, M

Granucci, F

Ricciardi-Castagnoli, P

Spreafico, A

Ackermann, M

Suter, M

机构：

[1] CNR, Cellular & Mol Pharmacol Ctr, I-20129 Milan, Italy

[2] Univ Zurich, Inst Virol, CH-8057 Zurich, Switzerland

[3] Univ Siena, Dipartimento Biol Mol, Policlin Le Scotte, I-53100 Siena, Italy

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1998年 / 213卷 / 02期

关键词：

rabbit immunoglobulins; phage display; Fab fragments; alkaline phosphatase; recombinant antibody;

D O I：

10.1016/S0022-1759(98)00029-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Rabbit monoclonal antibodies (RmAb) are not routinely obtained by eukaryotic cell fusion techniques. Therefore, we have applied phage display technology to produce a recombinant rabbit Fab molecule directed against the KLH model antigen. The Fab fragments selected from the rabbit phage display library were subcloned in an expression vector to permit the production of a fusion protein comprising a dimer of bacterial alkaline phosphatase (phoA). This fusion protein was directly produced into the periplasmic space of Escherichia coli. We show that a crude extract containing these conjugates can be used in a direct enzyme immunoassay, as exemplified in the case of the KLH antigen. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：201 / 212

页数：12

共 36 条

[1] HUMAN MONOCLONAL FAB FRAGMENTS DERIVED FROM A COMBINATORIAL LIBRARY BIND TO RESPIRATORY SYNCYTIAL VIRUS-F GLYCOPROTEIN AND NEUTRALIZE INFECTIVITY [J].