Molecular genetics of fructan metabolism in perennial ryegrass

被引:75
作者
Chalmers, J
Lidgett, A
Cummings, N
Cao, YY
Forster, J
Spangenberg, G [1 ]
机构
[1] La Trobe Univ, Ctr Plant Biotechnol, Primary Ind Res Victoria, Dept Primary Ind, Bundoora, Vic 3086, Australia
[2] La Trobe Univ, Mol Plant Breeding CRC, Bundoora, Vic 3086, Australia
关键词
fructan exohydrolase; fructan metabolism; fructosyltransferase; invertase; perennial ryegrass;
D O I
10.1111/j.1467-7652.2005.00148.x
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Fructans are the main storage carbohydrates of temperate grasses, sustaining regrowth immediately after defoliation, as well as contributing to the nutritive value of feed. Fructan metabolism is based on the substrate sucrose and involves fructosyltransferases (FTs) for biosynthesis and fructan exohydrolases (FEHs) for degradation. Sucrose is also utilized by invertases (INVs), which hydrolyse it into its constituent monosaccharides for use in metabolism. The isolation, molecular characterization, functional analysis, and phylogenetic relationships of genes encoding FTs, FEHs, and INVs from temperate grasses are reviewed, with an emphasis on perennial ryegrass (Lolium perenne L.). The roles these enzymes play in fructan accumulation and remobilization, and future biotechnological applications in molecular plant breeding are discussed.
引用
收藏
页码:459 / 474
页数:16
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