Evolution of phage with chemically ambiguous proteomes

被引:27
作者
Bacher, JM
Bull, JJ
Ellington, AD [1 ]
机构
[1] Univ Texas, Inst Mol & Cellular Biol, Austin, TX 78712 USA
[2] Univ Texas, Sect Integrat Biol, Austin, TX 78712 USA
[3] Univ Texas, Dept Chem & Biochem, Austin, TX 78712 USA
[4] Scripps Res Inst, Skaggs Inst Clin Biol, La Jolla, CA 92037 USA
关键词
D O I
10.1186/1471-2148-3-24
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Background: The widespread introduction of amino acid substitutions into organismal proteomes has occurred during natural evolution, but has been difficult to achieve by directed evolution. The adaptation of the translation apparatus represents one barrier, but the multiple mutations that may be required throughout a proteome in order to accommodate an alternative amino acid or analogue is an even more daunting problem. The evolution of a small bacteriophage proteome to accommodate an unnatural amino acid analogue can provide insights into the number and type of substitutions that individual proteins will require to retain functionality. Results: The bacteriophage Qbeta initially grows poorly in the presence of the amino acid analogue 6-fluorotryptophan. After 25 serial passages, the fitness of the phage on the analogue was substantially increased; there was no loss of fitness when the evolved phage were passaged in the presence of tryptophan. Seven mutations were fixed throughout the phage in two independent lines of descent. None of the mutations changed a tryptophan residue. Conclusions: A relatively small number of mutations allowed an unnatural amino acid to be functionally incorporated into a highly interdependent set of proteins. These results support the 'ambiguous intermediate' hypothesis for the emergence of divergent genetic codes, in which the adoption of a new genetic code is preceded by the evolution of proteins that can simultaneously accommodate more than one amino acid at a given codon. It may now be possible to direct the evolution of organisms with novel genetic codes using methods that promote ambiguous intermediates.
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页数:12
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