Cloning and characterization of a novel β integrin-related cDNA coding for the protein TIED ("ten β integrin EGF-like repeat domains") that maps to chromosome band 13q33:: A divergent stand-alone integrin stalk structure

被引:41
作者
Berg, RW
Leung, E
Gough, S
Morris, C
Yao, WP
Wang, SX
Ni, J
Krissansen, GW [1 ]
机构
[1] Univ Auckland, Fac Med & Hlth Sci, Dept Mol Med, Auckland, New Zealand
[2] Human Genome Sci Inc, Rockville, MD 20850 USA
[3] Christchurch Sch Med, Cytogenet & Mol Oncol Unit, Christchurch, New Zealand
基金
英国惠康基金;
关键词
D O I
10.1006/geno.1998.5707
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Herein we describe the cDNA sequence of a novel human gene, ITGBL1, encoding a beta integrin-related protein termed TIED [for ten beta integrin epidermal growth factor (EGF)-like repeat domains]. Overlapping cDNA clones from fetal lung, HUVEC, and osteoblast cDNA libraries encode a sequence comprising a typical signal peptide, followed by a hydrophilic 471-amino-acid domain containing 10 tandem EGF-like repeats strikingly similar to those found in the cysteine-rich "stalk-like" structure of integrin beta subunits, The EGF-like repeats of TIED and beta integrins are unique in that they alternate in homology and possess two additional cysteines (eight in total) whose positions differ from those in the other eight-cysteine EGF-like domains of laminin, fibrillin, and the latent TGF-beta binding proteins. TIED mRNA transcripts of 2.8 kb were detected in aorta, thymus, and osteogenic sarcoma cells. The ITGBL1 gene was mapped to human chromosome 13, band 13q33. We suggest that ITGBL1 may be linked in some way with the evolution of the integrin beta subunits. (C) 1999 Academic Press.
引用
收藏
页码:169 / 178
页数:10
相关论文
共 41 条
[1]  
ADAMS MD, 1995, NATURE, V377, P3
[2]   BASIC LOCAL ALIGNMENT SEARCH TOOL [J].
ALTSCHUL, SF ;
GISH, W ;
MILLER, W ;
MYERS, EW ;
LIPMAN, DJ .
JOURNAL OF MOLECULAR BIOLOGY, 1990, 215 (03) :403-410
[3]   Bovine PAS-6/7 binds alpha(V)beta(5) integrin and anionic phospholipids through two domains [J].
Andersen, MH ;
Berglund, L ;
Rasmussen, JT ;
Petersen, TE .
BIOCHEMISTRY, 1997, 36 (18) :5441-5446
[4]   STRUCTURE AND FUNCTION OF EPIDERMAL GROWTH FACTOR-LIKE REGIONS IN PROTEINS [J].
APPELLA, E ;
WEBER, IT ;
BLASI, F .
FEBS LETTERS, 1988, 231 (01) :1-4
[5]  
BOYD CD, 1988, AM J HUM GENET, V42, P309
[6]   EPIDERMAL GROWTH FACTOR-LIKE MODULES [J].
CAMPBELL, ID ;
BORK, P .
CURRENT OPINION IN STRUCTURAL BIOLOGY, 1993, 3 (03) :385-392
[7]  
CARRELL NA, 1985, J BIOL CHEM, V260, P1743
[8]  
CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2
[9]   FIBRILLIN BINDS CALCIUM AND IS CODED BY CDNAS THAT REVEAL A MULTIDOMAIN STRUCTURE AND ALTERNATIVELY SPLICED EXONS AT THE 5' END [J].
CORSON, GM ;
CHALBERG, SC ;
DIETZ, HC ;
CHARBONNEAU, NL ;
SAKAI, LY .
GENOMICS, 1993, 17 (02) :476-484
[10]   2 DISTINCT CELL ATTACHMENT SITES IN ENTACTIN ARE REVEALED BY AMINO-ACID SUBSTITUTIONS AND DELETION OF THE RGD SEQUENCE IN THE CYSTEINE-RICH EPIDERMAL GROWTH-FACTOR REPEAT-2 [J].
DONG, LJ ;
HSIEH, JC ;
CHUNG, AE .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (26) :15838-15843