Assessment of genetic diversity among selected groundnut germplasm. I: RAPD analysis

被引：55

作者：

Dwivedi, SL ^{[1
]}

Gurtu, S

Chandra, S

Yuejin, W

Nigam, SN

机构：

[1] Int Crops Res Inst Semi Arid Trop, Patancheru 502324, Andhra Pradesh, India

[2] Chinese Acad Agr Sci, Inst Crop Genet Resources, Beijing 100081, Peoples R China

来源：

PLANT BREEDING | 2001年 / 120卷 / 04期

关键词：

Arachis hypogaea; aflatoxin; genetic diversity; interspecific derivatives; RAPD;

D O I：

10.1046/j.1439-0523.2001.00613.x

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Assessment of genetic diversity in a crop species is prerequisite to its improvement. The use of germplasm with distinct DNA profiles will help to generate genetically diversified breeding populations. The aims of the present experiment were to study molecular diversity among selected groundnut accessions and identify those with distinct DNA profiles for mapping and genetic enhancement. Twenty-six accessions and eight primers of a 10-mer were selected for random amplified polymorphic DNA assay. The genetic similarity (S-ij) ranged from 59.0% to 98.8%, with an average of 86.2%. Both multidimensional scaling and unweighted pair-group method with arithmetic averages (UPGMA) dendrograms revealed the existence of five distinct clusters. However, this classification could not be related to known biological information about the accessions falling into different clusters. Some accessions with diverse DNA profiles (ICG 1448, 7101, and 1471, and ICGV 99006 and 99014) were identified for mapping and genetic enhancement in groundnut.

引用

页码：345 / 349

页数：5

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