Quantitative bioluminescence imaging of tumor-targeting bacteria in living animals

被引：131

作者：

Min, Jung-Joon ^{[1
,2
]}

Nguyen, Vu H. ^{[1
]}

Kim, Hyun-Ju ^{[3
]}

Hong, Yeongjin ^{[3
]}

Choy, Hyon E. ^{[3
]}

机构：

[1] Chonnam Natl Univ, Sch Med, Dept Nucl Med, Lab In Vivo Mol Imaging, Kwangju 501746, South Korea

[2] Gwangju Inst Sci & Technol, Bioimaging Res Ctr, Kwangju 500712, South Korea

[3] Chonnam Natl Univ, Sch Med, Dept Microbiol, Kwangju 501746, South Korea

来源：

NATURE PROTOCOLS | 2008年 / 3卷 / 04期

关键词：

D O I：

10.1038/nprot.2008.32

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe a protocol for imaging bacterial luciferase (Lux)-expressing bacteria in small living animals. In this protocol, light emitted by Lux-expressing bacteria is detected and monitored by a cooled charge-coupled device detector. When these bacteria are administered to animals, it provides a potentially valuable approach to generate sensitive whole-body images with extremely low background. This imaging technology should enable the real-time monitoring of bacterial migration into both primary and metastatic tumors in several different mouse tumor models at a strong quantification power.

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页码：629 / 636

页数：8

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