Elevated transforming growth factor-beta 1 (TGF-β1) levels in human fracture healing

被引：126

作者：

Sarahrudi, Kambiz ^{[1
]}

Thomas, Anita ^{[2
]}

Mousavi, Mehdi ^{[3
]}

Kaiser, Georg ^{[1
]}

Koettstorfer, Julia ^{[1
]}

Kecht, Mathias ^{[1
]}

Hajdu, S. ^{[1
]}

Aharinejad, S. ^{[2
]}

机构：

[1] Med Univ Vienna, Dept Traumatol, A-1090 Vienna, Austria

[2] Med Univ Vienna, Ctr Anat & Cell Biol, Lab Cardiovasc Res, A-1090 Vienna, Austria

[3] Danube Hosp, Dept Traumatol & Sportstraumatol, A-1220 Vienna, Austria

来源：

INJURY-INTERNATIONAL JOURNAL OF THE CARE OF THE INJURED | 2011年 / 42卷 / 08期

基金：

奥地利科学基金会;

关键词：

TGF-beta; 1; Bone fracture; Non-union; Delayed union; Human; Serum concentration; BONE DEFECT; DISTRACTION OSTEOGENESIS; SYSTEMIC REGULATION; CELL-PROLIFERATION; SKELETAL INJURY; REPAIR; EXPRESSION; GROWTH-FACTOR-BETA-1; DIFFERENTIATION; CHONDROGENESIS;

D O I：

10.1016/j.injury.2011.03.055

中图分类号：

R4 [临床医学];

学科分类号：

100218 [急诊医学];

摘要：

Introduction: Transforming growth factor-beta 1(TGF-beta 1) is a regulatory protein, involved in bone fracture healing. Circulating TGF-beta 1 levels have been reported to be a predictor of delayed bone healing and non-union, suggesting active relationship between tissue and circulating TGF-beta 1 in fracture healing. The purpose of this study was to analyse TGF-beta 1 local and serum concentrations in fracture healing to further contribute to the understanding of molecular regulation of fracture healing. Patients and methods: Serum samples of 113 patients with long bone fractures were collected over a period of 6 months following a standardised time schedule. TGF-beta 1 serum concentrations were measured using ELISA. Patients were assigned to 2 groups: Group 1 contained 103 patients with physiological healing. Group 2 contained 10 patients with impaired healing. Patients in both groups were matched. One patient of the group 2 had to be excluded because of missing match partner. In addition, fracture haematoma from 11 patients of group 1 was obtained to analyse local TGF-beta 1 concentrations. 33 volunteers donated serum which served as control. Results: TGF-beta 1 serum concentrations increased during the early healing period and were significantly higher in patients with physiological healing compared to controls (P = 0.04). Thereafter, it decreased continuously between weeks 2 and 8 and fell again after week 8. TGF-beta 1 serum concentrations in patients with physiological healing were significantly higher at week 24 compared to controls (P = 0.05). In non-unions, serum concentrations differed significantly from those of controls at week 6 (P = 0.01). No significant difference in between patients with physiological and impaired fracture healing was observed. Fracture haematoma contained significantly higher TGF-beta 1 concentrations than peripheral serum of the patients (P = 0.017). Conclusion: Elevated levels of TGF-beta 1 in haematoma and in serum after bone fracture especially during the entire healing process indicate its importance for fracture healing. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：833 / 837

页数：5

共 40 条

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