Visualization of ribosomal DNA loci in spore interphasic nuclei of glomalean fungi by fluorescence in situ hybridization

被引：132

作者：

Trouvelot, S ^{[1
]}

van Tuinen, D ^{[1
]}

Hijri, M ^{[1
]}

Gianinazzi-Pearson, V ^{[1
]}

机构：

[1] INRA, Lab Phytoparasitol, INRA, CNRS,Ctr Microbiol Sol & Environm, F-21034 Dijon, France

来源：

MYCORRHIZA | 1999年 / 8卷 / 04期

关键词：

arbuscular mycorrhiza; spore nuclei; fluorescence in situ hybridization; ribosomal DNA loci;

D O I：

10.1007/s005720050235

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Fluorescence in situ hybridization (FISH) was applied to interphasic nuclei isolated from spores of four species of AM fungi : Scutellospora castanea, Glomus mosseae, Glomus intraradices and Gigaspora rosea. Ribosomal DNA loci were visualized using digoxigenin-labeled 25 S rDNA probes obtained by nested PCR. Several hybridization sites were detected per nucleus and an internuclear variability was observed in the number of loci. This is the first report of successful application of FISH to analyse the genomes of glomalean fungi.

引用

页码：203 / 206

页数：4

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